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Zygomycota, previously classified as a distinct fungal group, are primarily terrestrial, saprophytic molds that play a crucial role as decomposers. Recent phylogenetic studies have revealed that these fungi are now divided into two major clades — Mucoromycota, which includes many symbiotic species, and Zoopagomycota, which primarily consists of parasitic and pathogenic fungi. These groups exhibit distinct ecological roles and reproductive strategies while sharing key structural and...
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Correction: Thaochan et al. Fungal-Infected Weeds: A Potential Source of Leaf Spot Disease in Rubber Trees from Southern Thailand. <i>J. Fungi</i> 2025, <i>11</i>, 220.

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Development of Metarhizium anisopliae as a Mycoinsecticide: From Isolation to Field Performance
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Identification of Mycoses in Developing Countries.

Amir Arastehfar1, Brian L Wickes2, Macit Ilkit3

  • 1Westerdijk Fungal Biodiversity Institute, 3584 CT Utrecht, The Netherlands. a.arastehfar@wi.knaw.nl.

Journal of Fungi (Basel, Switzerland)
|October 2, 2019
PubMed
Summary
This summary is machine-generated.

Accurate identification of fungal infections is crucial in developing countries where advanced diagnostics are limited. Innovative point-of-care tests, including CRISPR-based tools, offer promising alternatives for improved fungal diagnostics.

Keywords:
Nanopore sequencingmolecular toolsphenotypic assaysserology

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Area of Science:

  • Medical Mycology
  • Diagnostic Technologies
  • Global Health

Background:

  • Fungal infection diagnosis is challenging in developing countries due to limited access to advanced technologies.
  • Key antifungal treatments like echinocandins are unavailable, increasing the need for rapid and reliable diagnostics.
  • Barriers include cost, infrastructure, and personnel, hindering the adoption of high-precision methods.

Purpose of the Study:

  • To review current and emerging diagnostic technologies for fungal infections.
  • To highlight the importance of accessible and affordable diagnostic solutions for developing nations.
  • To explore the potential of novel technologies for point-of-care fungal diagnostics.

Main Methods:

  • Review of existing literature on fungal diagnostics.
  • Analysis of technological barriers in resource-limited settings.
  • Evaluation of point-of-care assays and emerging technologies like PCR and CRISPR.

Main Results:

  • Lateral flow assays for Aspergillus and Cryptococcus are relevant for developing countries.
  • PCR-based methods, including isothermal amplification, present alternatives to traditional techniques.
  • Next-generation sequencing and CRISPR-based tools show potential for future point-of-care applications.

Conclusions:

  • Developing countries require cost-effective and reliable fungal diagnostic methods.
  • Point-of-care tests and innovative technologies are essential for improving fungal infection management.
  • Further research into accessible diagnostics is critical for global health equity in mycology.