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Updated: Jan 6, 2026

Genome-Wide Analysis of DNA Methylation in Gastrointestinal Cancer
Published on: September 18, 2020
Aberrant DNA Hypermethylation Silenced LncRNA Expression in Gastric Cancer
Kuo-Wang Tsai1,2,3, Chung-Yu Tsai4, Nan-Hua Chou4
1Department of Medical Education and Research, Kaohsiung Veterans General Hospital, Kaohsiung, Taiwan, R.O.C. kwtsai6733@gmail.com hongtchang@gmail.com.
Background/Aim:
Long noncoding RNAs (lncRNAs) are noncoding transcripts that are >200 nucleotides in length. However, the biological functions and regulation mechanisms of lncRNAs in gastric carcinogenesis remain unknown.
Materials And Methods:
The expression levels of Linc00472 were analyzed by real-time PCR. The DNA methylation status was assessed using Combined Bisulfite Restriction Analysis (COBRA). The biological role of Linc00472 was assessed in AGS cells with Linc00472 overexpression.
Results:
Using the next-generation sequencing approach, we identified DNA methylation-associated lncRNAs in gastric cancer cells. Among them, the expression level of Linc00472 significantly decreased in gastric cancer tissues compared to adjacent normal tissues. Furthermore, we observed a more frequent hypermethylation of CpG islands upstream of Linc00472 in gastric cancer tissues. Ectopic Linc00472 expression could significantly inhibit gastric cancer cell growth and migration.
Conclusion:
Epigenetically regulated Linc00472 expression plays a crucial role in modulating gastric cancer cell growth and motility.
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