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Long Noncoding RNA GAS5 Acts As A Tumor Suppressor In Laryngeal Squamous Cell Carcinoma Via miR-21

Kexing Lyu1, Yang Xu1, Huijun Yue1

  • 1Department of Otolaryngology, The First Affiliated Hospital of Sun Yat-Sen University, Guangzhou, People's Republic of China.

Cancer Management and Research
|October 2, 2019
PubMed
Abstract

Insights

Long noncoding RNA GAS5 is downregulated in laryngeal squamous cell carcinoma (LSCC). Restoring GAS5 inhibits LSCC progression by targeting miR-21, suggesting its potential as a therapeutic target.

Area of Science:

  • Oncology
  • Molecular Biology
  • Genetics

Background:

  • Long noncoding RNAs (lncRNAs) play crucial roles in tumorigenesis.
  • Laryngeal squamous cell carcinoma (LSCC) is a significant health concern.
  • Understanding the function of specific lncRNAs in LSCC is vital for developing new therapies.

Purpose of the Study:

  • To investigate the role and biological function of lncRNA growth arrest-specific 5 (GAS5) in LSCC tumorigenesis.
  • To determine the expression levels of GAS5 in LSCC tissues and cell lines.
  • To explore the interaction between GAS5 and miR-21 in LSCC.

Main Methods:

  • Analysis of GAS5 expression in 59 LSCC tissues and adjacent samples using Real-time quantitative RT-PCR.
  • In vitro experiments involving ectopic expression of GAS5 in LSCC cell lines.
  • Cell proliferation (CCK8 assay) and apoptosis (Annexin V analysis) assays were performed.
  • Co-transfection of GAS5 and miR-21 to study their interaction and effects on BAX and CDK6 protein levels via Western blot.

Main Results:

  • GAS5 was significantly downregulated in LSCC tissues and cell lines.
  • Ectopic expression of GAS5 inhibited LSCC cell proliferation and promoted apoptosis.
  • GAS5 negatively correlated with miR-21 expression in LSCC.
  • Overexpression of miR-21 reversed the effects of GAS5 on cell apoptosis and proliferation, and modulated BAX and CDK6 expression.

Conclusions:

  • GAS5 suppresses LSCC progression by negatively regulating miR-21 and its downstream targets.
  • GAS5 functions as a tumor suppressor in LSCC.
  • GAS5 holds potential as a biomarker and therapeutic target for LSCC.
Keywords:
GAS5LSCCapoptosislong noncoding RNAmiR-21proliferation

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