Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Pore Transport and Ion-Pair Transport01:17

Pore Transport and Ion-Pair Transport

1.1K
Pore transport and ion-pair formation are critical mechanisms for the absorption and distribution of drugs in the body.
Pore transport, also known as convective transport, is a process where small molecules like urea, water, and sugars rapidly cross cell membranes as though there were channels or pores in the membrane. Although direct microscopic evidence is limited  but the concept of pores or channels is widely accepted based on physiological evidence. Despite the lack of direct...
1.1K
Protein Translocation Machinery on the ER Membrane01:28

Protein Translocation Machinery on the ER Membrane

6.4K
The translocon complex situated on the ER membrane is the main gateway for the protein secretory pathway. It facilitates the transport of nascent peptides into the ER lumen and their insertion into the ER membrane.
Sec61 protein conducting channel
In eukaryotes, the translocon complex comprises a core heterotrimeric translocator channel called the Sec61 complex. This channel includes three transmembrane proteins, Sec61α, Sec61β, and Sec61γ, and is the largest subunit of the...
6.4K
The Significance of Membrane Transport01:44

The Significance of Membrane Transport

40.9K
The transport of solutes across the cell membrane is essential for metabolic processes, like maintaining cell size and volume, generating the action potential, exchanging nutrients and gases, etc. Membrane transport can be either passive or active. It can be simple diffusion, facilitated, or mediated transport aided by transport proteins such as transporters and channels.
Transporters facilitate either an active or passive movement of solutes. They can allow a single-molecule transport down its...
40.9K
Energy to Drive Translocation01:37

Energy to Drive Translocation

2.6K
Mitochondrial protein import is powered by two distinct energy sources: ATP hydrolysis and electrochemical potential across the inner membrane. Newly synthesized precursors are bound by cytosolic chaperones of the Hsp70 family, which guide them to the import receptors on the mitochondrial surface. Utilizing the energy of ATP hydrolysis, Hsp70 chaperones transfer these precursors to the TOM receptors on the mitochondrial outer membrane.
Generally, polypeptides are unfolded by two distinct...
2.6K
Protein Transport into the Inner Mitochondrial Membrane01:34

Protein Transport into the Inner Mitochondrial Membrane

4.8K
Nuclear encoded mitochondrial precursors are imported to the inner membrane in a multistep process involving two separate translocons, TIM22 and TIM23. TIM23 is a cation-selective pore that remains closed by the N terminal segment of the protein. Negative charges on the TIM23 act as a receptor for the incoming precursor, pulling the positively charged matrix-targeting sequence for peptide insertion and translocation.
Transport of mitochondrial precursors across the TIM23 channel is driven by...
4.8K
Aquaporins01:25

Aquaporins

6.0K
Aquaporins or AQPs are a family of integral membrane proteins whose primary function is to transport water, while some called aquaglyceroporins also transport glycerol. In addition, aquaporins have also been suspected to be involved in transporting volatile substances, such as carbon dioxide and ammonia, across membranes. Such AQPs that act as gas channels are often highly expressed in cells involved in the gaseous exchange, such as red blood cells, epithelial cells, and pulmonary capillaries.
6.0K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Interface-Engineered Nanohybrid Membranes for Selective Boron Removal from Brackish Water and Seawater Reverse Osmosis Permeate.

Environmental science & technology·2026
Same author

Improved Lyrebird optimization for multi microgrid sectionalizing and cost efficient scheduling of distributed generation.

Scientific reports·2025
Same author

Multi-objective energy management in a renewable and EV-integrated microgrid using an iterative map-based self-adaptive crystal structure algorithm.

Scientific reports·2024
Same author

Optimizing dynamic economic dispatch through an enhanced Cheetah-inspired algorithm for integrated renewable energy and demand-side management.

Scientific reports·2024
Same author

Agent-Based Modeling of Microbial Communities.

ACS synthetic biology·2022
Same author

Dissipative Particle Dynamics Simulation of Nanoparticle Diffusion in a Crosslinked Polymer Network.

The journal of physical chemistry. B·2022
Same journal

From Cation Solvation to Anion Coordination: Lewis-Acidic Boranes Enable Halide Salt Electrolytes.

The journal of physical chemistry. B·2026
Same journal

In Vitro-Prepared A30P Alpha-Synuclein Fibrils Adopt the Conserved and Disease-Relevant Greek Key Fold.

The journal of physical chemistry. B·2026
Same journal

Metastructure Analysis of Self-Assembled Nanocubes with Different Equatorial Methyl Groups Based on Molecular Dynamics Simulations.

The journal of physical chemistry. B·2026
Same journal

A Cocoordinated <sup>1</sup>H Internal Reference Quantifies Proton-Exchange Bias in Coordinated-Water Diffusion.

The journal of physical chemistry. B·2026
Same journal

Unveiling Electrolyte-Dependent Coordination Site Dynamics for Redox Mediator Design in Lithium-O<sub>2</sub> Batteries: Exchange vs Rearrangement.

The journal of physical chemistry. B·2026
Same journal

The Role of Functional Groups in Substituted Benzoic Acids Used as Dopants in Liquid Crystal Mixtures on the Nematic-Isotropic Transitions.

The journal of physical chemistry. B·2026
See all related articles

Related Experiment Video

Updated: Jan 6, 2026

Membrane Transport Processes Analyzed by a Highly Parallel Nanopore Chip System at Single Protein Resolution
11:55

Membrane Transport Processes Analyzed by a Highly Parallel Nanopore Chip System at Single Protein Resolution

Published on: August 16, 2016

12.1K

Polyelectrolyte Translocation through a Tortuous Nanopore.

Karthik Nagarajan1, Shing Bor Chen1

  • 1Department of Chemical and Biomolecular Engineering , National University of Singapore , 117585 , Singapore.

The Journal of Physical Chemistry. B
|October 2, 2019
PubMed
Summary
This summary is machine-generated.

Tortuous nanopores significantly slow down DNA molecule movement, enabling accurate genetic information reading. This method enhances translocation control by inhibiting tension propagation for improved DNA sequencing applications.

More Related Videos

High Resolution Physical Characterization of Single Metallic Nanoparticles
09:56

High Resolution Physical Characterization of Single Metallic Nanoparticles

Published on: June 28, 2019

6.1K
Fine-tuning the Size and Minimizing the Noise of Solid-state Nanopores
09:43

Fine-tuning the Size and Minimizing the Noise of Solid-state Nanopores

Published on: October 31, 2013

14.1K

Related Experiment Videos

Last Updated: Jan 6, 2026

Membrane Transport Processes Analyzed by a Highly Parallel Nanopore Chip System at Single Protein Resolution
11:55

Membrane Transport Processes Analyzed by a Highly Parallel Nanopore Chip System at Single Protein Resolution

Published on: August 16, 2016

12.1K
High Resolution Physical Characterization of Single Metallic Nanoparticles
09:56

High Resolution Physical Characterization of Single Metallic Nanoparticles

Published on: June 28, 2019

6.1K
Fine-tuning the Size and Minimizing the Noise of Solid-state Nanopores
09:43

Fine-tuning the Size and Minimizing the Noise of Solid-state Nanopores

Published on: October 31, 2013

14.1K

Area of Science:

  • Nanotechnology
  • Biophysics
  • Computational Science

Background:

  • Nanopore technology offers potential for DNA sequencing.
  • High translocation rates impede accurate genetic information retrieval.
  • Controlling polyelectrolyte translocation speed is crucial for sequencing.

Purpose of the Study:

  • To investigate the use of tortuous nanopores for controlling polyelectrolyte translocation rates.
  • To understand how pore geometry influences translocation dynamics.
  • To explore simulation methods incorporating hydrodynamic, electrostatic, and electric field effects.

Main Methods:

  • Dissipative particle dynamics simulations were utilized.
  • Simulations incorporated hydrodynamic and electrostatic interactions.
  • Spatial variations in electric field strength were considered.

Main Results:

  • Translocation time (⟨τ⟩) increased with pore length and tortuosity.
  • Translocation time decreased with increasing pore width.
  • Tortuosity increased translocation time by up to 187% for the longest pore.
  • Slower translocation in tortuous pores is attributed to inhibited tension propagation.

Conclusions:

  • Tortuous nanopores effectively control polyelectrolyte translocation rates.
  • Pore geometry, specifically tortuosity, is a key factor in regulating translocation speed.
  • This approach offers a viable strategy for enhancing DNA sequencing accuracy.