Mass Spectrometry-Based Plasma Proteomics: Considerations from Sample Collection to Achieving Translational Data.

Area of Science:

• Biochemistry
• Clinical Proteomics
• Human Biology

Background:

• Plasma proteomics is a promising field for clinical translation.
• Challenges include biological variability, sample handling, and technological limitations.
• The Human Plasma Proteome Project (HPPP) addresses these challenges.

Purpose of the Study:

• To provide recommendations for plasma proteomics.
• To guide study design, sample collection, and data analysis.
• To facilitate the clinical application of plasma proteomics.

Main Methods:

• Consensus recommendations from the Human Plasma Proteome Project (HPPP).
• Focus on study design, plasma collection, and processing.
• Guidelines for mass spectrometry (MS) data acquisition and analysis.

Main Results:

• Detailed considerations for each step of the plasma proteomics pipeline.
• Emphasis on quality metrics and bioinformatic approaches.
• Strategies to overcome interindividual and sample variability.

Conclusions:

• Standardized approaches are crucial for reliable plasma proteomics.
• Informed analysis accelerates clinical integration of proteomics.
• This work supports the advancement of plasma proteomics for health and disease research.