Related Experiment Video
Updated: Jan 6, 2026

Comparing Metastatic Clear Cell Renal Cell Carcinoma Model Established in Mouse Kidney and on Chicken Chorioallantoic Membrane
Published on: February 8, 2020
Oleic acid promotes cell invasion through an integrin-linked kinase signaling pathway in renal cell carcinoma
Zhen-Hua Liu1, Cai-Peng Qing2, Zheng-Zuo Sheng3
1Department of Urology, Beijing JiShuiTan Hospital, the 4th Medical College of Peking University, Beijing, China.
Abstract:
The change of fatty acid composition has been regarded as an indicator of altered lipid metabolism during human tumourigenesis, but the details are still unclear. We have previously demonstrated a monounsaturated fatty acid (MUFA) named oleic acid (OA) was involved in renal cell carcinoma (RCC) cell growth, as an extracellular signaling molecule to regulate 786-O cell proliferation via the integrin-linked kinase (ILK) pathway. In this study, we further observe the effects of OA on cell invasion of RCC and the potential mechanism by which OA worked was determined. The transwell invasion assay showed OA increased cell invasion of RCC in a dose-dependent manner. Western blotting results indicated ILK, COX-2, and MMP-9 proteins were involved for their high expressions and these effects were reversed when down-regulating the expression of ILK by special siRNA. The MMPs inhibitor GM6001 could weaken the abilities of OA on RCC cells invasion. These results suggested MUFA indeed affected cell invasion of RCC, which was depended by the regulation of ILK pathway.
Related Concept Videos
Intracellular Signaling Affects Focal Adhesions
Some...
mTOR Signaling and Cancer Progression
The mTOR pathway or the...
Activation of Integrins
In "outside-in signaling," external factors in the extracellular space bind to exposed ligand binding sites on integrins. This causes the inactive protein to undergo a conformational change to become active. Integrins are often clustered on the cell membrane. Repetitive and regularly spaced ligand binding...
PI3K/mTOR/AKT Signaling Pathway
Cancer Cell Migration through Invadopodia
Canonical Wnt Signaling Pathway

