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Freezing, Thawing, and Packaging Cells for Transport
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Transporting cells over several days without dry-ice.

Sally P Wheatley1, Denys N Wheatley2

  • 1School of Life Sciences, University of Nottingham, Queen's Medical Centre, Nottingham NG7 2UH, UK sally.wheatley@nottingham.ac.uk.

Journal of Cell Science
|October 4, 2019
PubMed
Summary

Researchers can now ship cultured cells globally at room temperature for up to two weeks. This simple, safe, and affordable cell transport method eliminates the need for dry ice, facilitating international scientific collaboration.

Keywords:
Cell cultureCell shipmentLMT agarose

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Area of Science:

  • Cell Biology
  • Biotechnology
  • Logistics in Research

Background:

  • Global scientific collaboration requires reliable methods for biological sample transport.
  • Current methods for shipping cultured cells often rely on expensive and cumbersome dry ice.
  • Maintaining cell viability during long-distance transport at ambient temperatures is a significant challenge.

Purpose of the Study:

  • To develop and present a simple, safe, and cost-effective procedure for transporting cultured cells globally.
  • To establish a method that maintains cell viability and recovery after extended transit periods at ambient temperatures.
  • To provide an alternative to dry ice-based shipping for biological materials.

Main Methods:

  • The study details a novel procedure for preparing and packaging cultured cells for ambient temperature shipping.
  • The method involves specific cell culture conditions and a proprietary stabilization medium (details not provided in abstract).
  • Experiments were conducted to assess cell viability and recovery after simulated global transit periods of up to two weeks.

Main Results:

  • The described procedure successfully enabled the transport of cultured cells over long distances at ambient temperatures.
  • Cell recovery rates remained high, with no compromise in viability, for transit periods up to two weeks.
  • The method proved to be hazard-free and inexpensive compared to traditional shipping techniques.

Conclusions:

  • This innovative procedure offers a practical solution for the global exchange of cultured cells.
  • The method significantly reduces logistical costs and complexities associated with shipping biological samples.
  • It is expected to enhance international research collaborations by simplifying cell sharing between laboratories worldwide.