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Related Experiment Video

Updated: Jan 6, 2026

Simultaneous Interference Reflection and Total Internal Reflection Fluorescence Microscopy for Imaging Dynamic Microtubules and Associated Proteins

Simultaneous Interference Reflection and Total Internal Reflection Fluorescence Microscopy for Imaging Dynamic Microtubules and Associated Proteins

Published on: May 3, 2022

Fast objective coupled planar illumination microscopy.

Cody J Greer¹, Timothy E Holy²

¹Department of Neuroscience, Washington University in Saint Louis, Saint Louis, MO, 63110, USA. cody.greer@wustl.edu.

Nature Communications

|October 4, 2019

Summary

This summary is machine-generated.

Objective Coupled Planar Illumination (OCPI) microscopy enhances speed for 3D biological imaging. Distributed Planar Imaging (DPI) overcomes camera limits, enabling faster fluorescence microscopy of large volumes.

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Last Updated: Jan 6, 2026

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Simultaneous Multicolor Imaging of Biological Structures with Fluorescence Photoactivation Localization Microscopy

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Published on: December 9, 2013

Area of Science:

Biophysics
Optical Imaging
Microscopy

Background:

Light sheet fluorescence microscopy is ideal for high-speed 3D biological dynamics.
Existing light sheet techniques are limited by volume scanning rate and camera speed.
Faster imaging is crucial for capturing rapid biological processes.

Purpose of the Study:

To present speed-optimized Objective Coupled Planar Illumination (OCPI) microscopy.
To introduce Distributed Planar Imaging (DPI) to overcome camera speed limitations.
To demonstrate advanced fluorescence microscopy for fast biological processes.

Main Methods:

Developed speed-optimized Objective Coupled Planar Illumination (OCPI) microscopy.
Implemented Distributed Planar Imaging (DPI) to parallelize image acquisition across multiple cameras.
Performed fast calcium imaging in larval zebrafish brains.

Main Results:

OCPI microscopy achieves 40 Hz imaging of 700 μm-thick volumes with sufficient camera speed.
DPI effectively addresses camera speed limitations in light sheet microscopy.
A heartbeat-induced artifact in zebrafish brain imaging was identified and shown to be removable above 15 Hz.

Conclusions:

Speed-optimized OCPI microscopy and DPI significantly enhance fluorescence microscopy capabilities.
These advancements enable monitoring of fast biological processes in large volumes.
The developed techniques extend the application range of light sheet microscopy for dynamic biological studies.