A Novel System to Discriminate HLA-C mir148a Binding Site by Allele-Specific Quantitative PC R
Priscilla Biswas1, Eddi Di Marco2, Mauro S Malnati3
1Unit of Human Virology, Division of Immunology, Transplantation and Infectious Diseases, IRCCS Ospedale San Raffaele, Milan, Italy.
Methods in Molecular Biology (Clifton, N.J.)
|October 4, 2019
Abstract:
The levels of expression of the HLA-class I molecules are critical for modulating T/NK lymphocytes effector functions. Among HLA molecules, HLA-C, the most recent developed form of class I antigens, is subjected to multiple post transcriptional level of regulation that affect its cell surface expression.We describe a new method of allele-specific real-time PCR that monitor the integrity/disruption of the binding site of the microRNA Hsa-miR-148a, a key factor associated to the levels of HLA-C expression in the Caucasian populations.
