Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Real Time RT-PCR02:57

Real Time RT-PCR

64.3K
Real-time reverse transcription-polymerase chain reaction, or Real-time RT-PCR, is an analytical tool used to determine the expression level of target genes. The method involves converting mRNA to complementary DNA with the help of an enzyme known as reverse transcriptase, followed by the PCR amplification of the cDNA. These two processes can be performed simultaneously in a single tube or separately as a two-step reaction.
The real-time quantification of the number of amplified products is...
64.3K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Poisson Plus Quantification for Digital PCR Systems.

Scientific reports·2017
Same author

Tumor burden monitoring using cell-free tumor DNA could be limited by tumor heterogeneity in advanced breast cancer and should be evaluated together with radiographic imaging.

BMC cancer·2017
Same author

Digital PCR modeling for maximal sensitivity, dynamic range and measurement precision.

PloS one·2015
Same author

Temporal sequence and cell cycle cues in the assembly of host factors at the yeast 2 micron plasmid partitioning locus.

Nucleic acids research·2013

Related Experiment Video

Updated: Jan 6, 2026

Simple Bulk Readout of Digital Nucleic Acid Quantification Assays
06:55

Simple Bulk Readout of Digital Nucleic Acid Quantification Assays

Published on: September 24, 2015

8.6K

Digital PCR and the QuantStudio™ 3D Digital PCR System.

Marion Laig1, Christie Fekete2, Nivedita Majumdar2

  • 1Thermo Fisher Scientific, South San Francisco, CA, USA. marion.laig@thermofisher.com.

Methods in Molecular Biology (Clifton, N.J.)
|October 4, 2019
PubMed
Summary

Digital PCR offers unparalleled precision for genetic material detection and quantification. This technology, exemplified by the QuantStudio 3D system, has diverse applications from rare mutation detection to copy number variation analysis.

Keywords:
Copy numberDigital PCRGMOLimit of detectionPoissonRare mutation

More Related Videos

Detection of a CDH1 Rare Transcript Variant in Fresh-frozen Gastric Cancer Tissues by Chip-based Digital PCR
09:16

Detection of a CDH1 Rare Transcript Variant in Fresh-frozen Gastric Cancer Tissues by Chip-based Digital PCR

Published on: February 5, 2018

6.5K
Quantification of Circular RNAs Using Digital Droplet PCR
08:39

Quantification of Circular RNAs Using Digital Droplet PCR

Published on: September 16, 2022

4.0K

Related Experiment Videos

Last Updated: Jan 6, 2026

Simple Bulk Readout of Digital Nucleic Acid Quantification Assays
06:55

Simple Bulk Readout of Digital Nucleic Acid Quantification Assays

Published on: September 24, 2015

8.6K
Detection of a CDH1 Rare Transcript Variant in Fresh-frozen Gastric Cancer Tissues by Chip-based Digital PCR
09:16

Detection of a CDH1 Rare Transcript Variant in Fresh-frozen Gastric Cancer Tissues by Chip-based Digital PCR

Published on: February 5, 2018

6.5K
Quantification of Circular RNAs Using Digital Droplet PCR
08:39

Quantification of Circular RNAs Using Digital Droplet PCR

Published on: September 16, 2022

4.0K

Area of Science:

  • Molecular Biology
  • Genetics
  • Biotechnology

Background:

  • Digital PCR (polymerase chain reaction) offers high precision for genetic analysis.
  • Accurate quantification and detection of genetic material are crucial in various biological applications.

Purpose of the Study:

  • To explain the fundamentals of digital PCR technology and its modeling using Poisson statistics.
  • To showcase a successful implementation of digital PCR using the QuantStudio 3D system.
  • To review the broad range of applications for digital PCR.

Main Methods:

  • Explanation of digital PCR principles and Poisson statistical modeling.
  • Demonstration of the Applied Biosystems QuantStudio 3D digital PCR system.
  • Review of existing literature and case studies on digital PCR applications.

Main Results:

  • Digital PCR enables highly precise measurements for genetic material detection and quantification.
  • The QuantStudio 3D system provides a robust platform for digital PCR implementation.
  • Digital PCR is applicable to a wide array of fields, including liquid biopsy, rare mutation detection, and copy number variation analysis.

Conclusions:

  • Digital PCR is a powerful tool for applications requiring high precision in genetic analysis.
  • Its versatility makes it suitable for both rare target detection and accurate quantification.
  • The technology is poised to significantly impact fields like diagnostics, research, and quality control.