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Exploring the expression and preliminary function of chicken Gimap5 gene
Wanting Zhang1, Sifan Xu1, Guanxian Wu1
1College of Life Science and Technology, Harbin Normal University, Harbin, China.
Peerj
|October 4, 2019
Summary
The chicken GTPase immune-associated protein 5 (Gimap5) gene was cloned and characterized. Gimap5 shows varied expression across tissues and developmental stages, playing a role in immune responses.
Area of Science:
- Immunology
- Genetics
- Molecular Biology
Background:
- GTPase immune-associated protein 5 (Gimap5) is crucial for T cell homeostasis and immune tolerance.
- No prior studies have investigated the chicken Gimap5 gene.
Purpose of the Study:
- To clone and characterize the chicken Gimap5 gene.
- To analyze its tissue expression patterns during development and immune responses.
Main Methods:
- Cloning of the full-length cDNA sequence of chicken Gimap5.
- Analysis of Gimap5 gene transcription in various tissues at different developmental stages.
- Evaluation of Gimap5 gene expression in immune-related tissues during immune responses.
Main Results:
- The chicken Gimap5 cDNA is 771 bp, encoding a 256-amino acid protein.
- Gimap5 is transcribed in multiple tissues, with expression increasing during development and peaking in liver and large intestine at 10 months.
- Immune challenge resulted in differential Gimap5 regulation: down-regulated in liver, spleen, and bursa of Fabricius, but up-regulated in the thymus.
Conclusions:
- Chicken Gimap5 is a multifunctional gene involved in tissue development and immune responses.
- The findings provide a foundation for further research into Gimap5's role in poultry immunity.
