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Updated: Jan 6, 2026

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Fluorescent 2-(Pyridin-2-yl)vinyl Pyridine Dyes and Their Thermocontrolled Release
Agnieszka Witkowska1, Jolanta Brzezinska1, Tomasz Pedzinski2
1Institute of Bioorganic Chemistry , Polish Academy of Sciences , Noskowskiego 12/14, 61-704 Poznań , Poland.
Abstract:
The generation of unique thermosensitive fluorescent dyes via heteroaromatic Heck cross-coupling and N-pyridin-2-yl nucleophilic substitution was described. To demonstrate thermosensitive properties, the precursor was converted into carbonates or phosphates and heated at various temperatures and for various time periods. Significant changes in the fluorescence intensity and emission wavelengths, between carbonates and the cyclic product, were observed, and it was proved that the dyes may serve as removable fluorescent labels with large Stokes shifts (>80 nm). The application of thermosensitive fluorescent dyes in oligonucleotide labeling has been demonstrated.
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