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A ribonuclease-dependent cleavable beacon primer triggering DNA amplification for single nucleotide mutation
Xiong Ding1, Kun Yin1, Ju Chen2
1Department of Biomedical Engineering, University of Connecticut Health Center, Farmington, Connecticut 06030, USA. chaliu@uchc.edu.
Abstract:
We described a ribonuclease-dependent cleavable beacon primer, an energy-transfer-tagged oligonucleotide inserted with a ribonucleotide, which can be cleaved by ribonuclease to generate enhanced fluorescence signals and initiate DNA amplification for single nucleotide mutation detection with ultrahigh sensitivity and selectivity.
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