Validation of a simple extraction procedure for bisphenol A identification from human plasma

Area of Science:

• Environmental Health
• Analytical Chemistry
• Toxicology

Background:

• General population exposure to bisphenol A (BPA) is ubiquitous via food packaging, plastics, and personal care products.
• Detectable BPA levels are found in 91-99% of the population.
• Existing methods for BPA quantification can be costly and time-consuming.

Purpose of the Study:

• To develop an inexpensive, rapid, and simple protein precipitation method for BPA extraction from human plasma.
• To validate the method for accurate and precise quantification of BPA in large sample sets.
• To assess BPA levels in a cohort of healthy volunteers and identify potential demographic correlations.

Main Methods:

• Protein precipitation procedure for BPA extraction from human plasma.
• Liquid chromatography-tandem mass spectrometry (LC-MS/MS) for BPA analysis.
• Analysis of plasma samples from 150 healthy volunteers.

Main Results:

• The developed method is accurate, precise, and reproducible for BPA quantification in the range of 10-2000 ng/mL.
• The mean observed BPA level in the study population was 2.22 ± 9.91 ng/mL.
• Higher BPA levels were significantly associated with females (p=0.002), individuals aged 33+ (p=0.000), and tap water consumption (p=0.005).

Conclusions:

• An efficient and economical method for BPA quantification in human plasma has been established.
• The method is suitable for risk assessment in large and diverse populations.
• Demographic factors including sex, age, and drinking water source are associated with varying BPA levels.