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Methods to Quantify DNA Transfer in Enterococcus.

Carla Novais1, Ana R Freitas2, Ricardo León-Sampedro3

  • 1Laboratory of Microbiology, Biological Sciences Department, Faculty of Pharmacy, UCIBIO/REQUIMTE, University of Porto, Porto, Portugal. casilva@ff.up.pt.

Methods in Molecular Biology (Clifton, N.J.)
|October 5, 2019
PubMed
Summary

This study details conjugation protocols for quantifying DNA transfer in Enterococcus, a key process in genetic research. It revises current lab strains and perspectives on bacterial DNA exchange.

Keywords:
ConjugationConjugative transposons (CTn)EnterococcusHorizontal gene transfer (HGT)Integrative conjugative elements (ICEs)Plasmids

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Area of Science:

  • Microbiology
  • Molecular Genetics
  • Bacterial Conjugation

Background:

  • DNA uptake in Enterococcus typically occurs via conjugation.
  • Conjugation is vital for studying mobile genetic elements and plasmid host ranges.
  • Artificial transformation methods like electroporation are common in molecular genetics.

Purpose of the Study:

  • To describe conjugation protocols for quantifying DNA transfer in Enterococcus.
  • To revise current perspectives on Enterococcus DNA transfer.
  • To update information on commonly used laboratory strains for Enterococcus research.

Main Methods:

  • Detailed description of conjugation protocols.
  • Quantification of DNA transfer efficiency.
  • Revision of existing laboratory strains and methodologies.

Main Results:

  • Established protocols for quantifying DNA transfer in Enterococcus via conjugation.
  • Provided an updated perspective on Enterococcus conjugation and lab strains.
  • Highlighted the importance of conjugation in genetic research.

Conclusions:

  • Conjugation protocols are essential for studying DNA transfer in Enterococcus.
  • Updated strain information and perspectives enhance research reliability.
  • This work complements previously described electrotransformation protocols.