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Methods for the Analysis and Characterization of Defense Mechanisms Against Horizontal Gene Transfer: CRISPR Systems

Alicia Calvo-Villamañán1,2, Aude Bernheim1,2,3,4, David Bikard5

  • 1Synthetic Biology Group, Microbiology Department, Institut Pasteur, Paris, France.

Methods in Molecular Biology (Clifton, N.J.)
|October 5, 2019
PubMed

Keywords:
AdaptationAdaptive immunityCRISPR-CasHorizontal gene transfer (HGT)InterferenceMobile genetic elements (MGE)ProtospacerProtospacer adjacent motif (PAM)Spaceradaptive immunitybacterial geneticsRNA-guided nucleasesphage resistance

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