Formation of partial-denitrification (PD) granular sludge from low-strength nitrate wastewater: The influence of...

Granular sludge has been believed to be a promising technology in wastewater biological treatment. However, the formation of granules at low substrate concentration is a difficult task that has seldom been achieved. This study aimed at forming the granules in the recently developed partial-denitrification (PD, NO₃^--N→NO₂^--N) for nitrite production. Two sequencing batch reactors (SBRs) were operated at a low nitrate of 30 mg N/L with nitrate loading rate (NLR) of 0.12 (R1) and 0.24 kg N/m³/d (R2). Results showed that the granulation of PD sludge experienced a developing and matured process with the progressive increase in size followed by maintaining a stable value. Higher NLR resulted in a more rapid granulation with the larger and looser structure. While the granules under lower NLR appeared to be denser and more compact with better settling ability. Microbial communities of two SBRs were revealed to show little difference, with the PD functional bacteria of Thauera (50.7% in R1 and 55.4% in R2) dominated during the granulation process. The Flavobacterium, likely to be closely related with sludge granulation, accounted for a higher proportion in R2 (10.16%) than R1 (5.91%), which might result in a larger granule formed in R2. This study clearly confirmed the feasibility of granulation of PD sludge under low nitrogen loads, shedding new light on the low-strength nitrate wastewater treatment with an efficient and economical way.