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sncRNAs packaged by Helicobacter pylori outer membrane vesicles attenuate IL-8 secretion in human cells

Hongxia Zhang1, Yingxuan Zhang2, Zifan Song2

  • 1Department of Medical Microbiology, School of Medicine, Nanchang University, Nanchang, 330006, China.

International Journal of Medical Microbiology : IJMM
|October 6, 2019
PubMed

Insights

Helicobacter pylori outer membrane vesicles (OMVs) deliver small noncoding RNAs (sncRNAs) to human cells, modulating immune responses. These sncRNAs in OMVs help H. pylori evade host immunity.

Area of Science:

  • Microbiology
  • Immunology
  • Molecular Biology

Background:

  • Bacterial outer membrane vesicles (OMVs) mediate host-pathogen communication.
  • OMVs contain small noncoding RNAs (sncRNAs) that can regulate host mRNA function and immune responses.

Purpose of the Study:

  • To identify differentially packaged sncRNAs in Helicobacter pylori OMVs.
  • To investigate the transfer of these OMV sncRNAs to human gastric cells.
  • To determine the role of these sncRNAs in modulating host immune responses.

Main Methods:

  • Isolation and characterization of OMVs from H. pylori.
  • Identification and quantification of sncRNAs within OMVs.
  • Co-culture of human gastric adenocarcinoma cells (AGS cells) with H. pylori OMVs.
  • Measurement of interleukin-8 (IL-8) secretion in response to OMV treatment.

Main Results:

  • Two specific sncRNAs (sR-2509025 and sR-989262) were found to be enriched in H. pylori OMVs.
  • These OMV sncRNAs were successfully transferred to AGS cells.
  • The presence of these sncRNAs reduced lipopolysaccharide or OMV-induced IL-8 secretion from AGS cells.

Conclusions:

  • sncRNAs packaged within H. pylori OMVs play a role in host-pathogen interactions.
  • H. pylori utilizes OMV-delivered sncRNAs to evade the host immune response.
  • This represents a novel mechanism of immune evasion by H. pylori.
Keywords:
H.PyloriIL-8Outer membrane vesiclesSmall noncoding RNAs (sncRNAs)

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