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Membrane proteomic analysis reveals the intestinal development is deteriorated by intrauterine growth restriction in
Shimeng Huang1, Cong Liu1, Na Li1
1State Key Laboratory of Animal Nutrition, College of Animal Science and Technology, China Agricultural University, Beijing, 100193, China.
Insights
Intrauterine growth restriction (IUGR) significantly alters the jejunal membrane proteome in piglets, impacting functions like nutrient transport and oxidative stress response during early development.
Area of Science:
- Gastroenterology
- Proteomics
- Neonatal Development
Background:
- Intrauterine growth restriction (IUGR) is associated with intestinal proteome alterations.
- The specific impact of IUGR on the neonatal intestinal membrane proteome remains largely uncharacterized.
Purpose of the Study:
- To investigate the effects of IUGR on the jejunal mucosal membrane proteome in piglets during neonatal development.
- To identify differentially expressed membrane proteins (DEMPs) and their functional implications in IUGR piglets.
Main Methods:
- Utilized iTRAQ-based proteomics technology and biochemical analysis.
- Screened 361 membrane proteins and identified DEMPs at days 0, 7, and 21 post-birth.
Main Results:
- Identified 8, 5, and 1 DEMPs at days 0, 7, and 21, respectively.
- Observed reduced levels of proteins involved in dopamine receptor binding, transmembrane transport, signal transduction, and GLUT4 translocation.
- Noted increased abundance of proteins related to autophagy, vitamin metabolism, and intracellular protein transport.
- Found decreased levels of proteins crucial for response to oxidative stress in IUGR piglets.
Conclusions:
- IUGR profoundly impacts the jejunal membrane proteome during neonatal development.
- Altered protein expression affects critical functions including nutrient absorption, cellular signaling, and oxidative stress defense.
- These proteomic changes highlight potential mechanisms underlying the long-term health consequences of IUGR.
Abstract:
The alterations of the intestinal proteome were observed in intrauterine growth restriction (IUGR) piglets during early life by gel-based approaches. Nevertheless, how IUGR affects the intestinal membrane proteome during neonatal development remains unclear. Here, we applied the iTRAQ-based proteomics technology and biochemical analysis to investigate the impact of IUGR on the membrane proteome of the jejunal mucosa in the piglets. Three hundred sixty-one membrane proteins were screened by functional prediction. Among them, eight, five, and one differentially expressed membrane proteins were identified between IUGR and NBW piglets at day 0, day 7, and day 21 after birth, respectively. Differentially expressed membrane proteins (DEMPs) including F1SBL3, F1RRW8, F1S539, F1S2Z2, F1RIR2, F1RUF2 I3LP60, Q2EN79, and F1SIH8 were reduced while the relative abundance of I3L6A2, F1SCJ1, F1RI18, I3LRJ7, and F1RNN0 were increased in IUGR piglets than NBW piglets. From the aspects of function, F1RRW8, F1S539, F1S2Z2, and F1RIR2 are mainly associated with D2 dopamine receptor binding, transmembrane transport of small molecules, signal transduction, and translocation of GLUT4, respectively, and F1SIH8, I3LRJ7, and F1RNN0 are related to autophagy, metabolism of vitamins, and intracellular protein transport. Additionally, IUGR decreased the level of proteins (F1RRW8, Q2EN79, and F1RI18) that are involved in response to oxidative stress.
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