Related Experiment Video

Updated: Aug 18, 2026

Study of Endoplasmic Reticulum and Mitochondria Interactions by In Situ Proximity Ligation Assay in Fixed Cells
09:34

Study of Endoplasmic Reticulum and Mitochondria Interactions by In Situ Proximity Ligation Assay in Fixed Cells

Published on: December 10, 2016

Site specificity in vimentin-membrane interactions: intermediate filament subunits associate with the plasma membrane

S D Georgatos, D C Weaver, V T Marchesi

    The Journal of Cell Biology
    |June 1, 1985
    PubMed

    More Related Videos

    In Vitro Polymerization of F-actin on Early Endosomes12:15

    In Vitro Polymerization of F-actin on Early Endosomes

    Published on: August 28, 2017

    In Vitro Reconstitution of the Actin Cytoskeleton Inside Giant Unilamellar Vesicles10:19

    In Vitro Reconstitution of the Actin Cytoskeleton Inside Giant Unilamellar Vesicles

    Published on: August 25, 2022

    Related Concept Videos

    Membrane Domains01:18

    Membrane Domains

    The membrane domains concentrate specific lipids and proteins at one place within the membrane, which helps in cell signaling, adhesion, and other critical cellular processes. These domains can differ in size, composition, function, and lifespan.
    Protein Domains
    The membrane comprises a group of distinct proteins responsible for carrying out a cell's specific function. For example, the plasma membrane of the human sperm, or a single germ cell, contains a unique set of proteins in the anterior...
    The Structure of Intermediate Filaments01:19

    The Structure of Intermediate Filaments

    The intermediate filaments are one of three widely studied cytoskeletal filaments. They are so named as their diameter (10 nm) is in between that of microfilaments (7 nm) and the microtubules (25 nm).  These filaments are highly stable and can remain intact when exposed to high salt concentrations and detergents. These filaments are responsible for providing stability and mechanical support to the cells. They also help in cell adhesion and maintaining tissue integrity.
    Intermediate filaments...
    Formation of Intermediate Filaments00:57

    Formation of Intermediate Filaments

    Intermediate filaments are cytoskeletal proteins with higher tensile strength and flexibility than microfilaments and microtubules. Unlike the other two cytoskeletal proteins, intermediate filament formation lacks the enzymatic activity to hydrolyze nucleotides like ATP and GTP to generate energy for polymerization. Therefore, the formation of intermediate filaments is multistep self-assembly. The involvement of any accessory proteins in intermediate filament formation has not yet been reported.
    Mechanisms of Membrane Domain Formation00:59

    Mechanisms of Membrane Domain Formation

    Different physical properties of lipids and proteins allow them to localize and form distinct islands or domains in the membrane. Some membrane domains are formed due to protein-protein interactions, whereas others are formed due to the presence of specific lipids such as sphingolipids and sterols—for example, large proteins, such as bacteriorhodopsin, aggregate and create distinct domains.
    Another mechanism for membrane domain formation involves membrane proteins interacting with cytoskeletal...
    Disassembly of Intermediate Filaments01:35

    Disassembly of Intermediate Filaments

    Intermediate filaments (IFs) do not undergo spontaneous disassembly. Enzymes, kinases, and phosphatases add and remove phosphates from specific sites to regulate their disassembly. The IF concentration in the cytoplasm also regulates the disassembly. If the concentration crosses a threshold, it activates the protein kinases in the vicinity, allowing the phosphorylation of IFs.
    Keratin proteins, found at the cell periphery near cell junctions, undergo a cycle of assembly and disassembly. In Type...
    Anchoring Junctions01:03

    Anchoring Junctions

    Anchoring junctions are multiprotein complexes that help cells connect to other cells and the extracellular matrix. Anchoring junctions are present on the lateral and basal surfaces of cells, providing strong and flexible connections. Focal adhesions are often formed due to cell interactions with the ECM substrata, which initiate signal transduction via kinase cascades and other mechanisms. Together, they provide stability and tissue integrity. There are three types of anchoring junctions:...