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An experimental mouse model for phage therapy of bacterial pathogens causing bacteremia
Sadeq Abdulridha Gatea Kaabi1, Hadeel Kareem Musafer1
1Department of Biology, College of Science, Mustansiriyah University, Box 14022, Baghdad, Iraq.
Abstract:
A minimum lethal dose on mice for five bacterial pathogens were identified with PCR of neonatal septicemia of Escherichia coli, Klebsiella pneumoniae, Haemophilus influenzae, Pseudomonas aeruginosa, Citrobacter freundii and Moraxella catarrhalis were studied. Bacteriophage preparations active against 10 isolates of E. coli, 10 isolates of K. pneumoniae, 4 isolates of H. influenzae, 3 isolates of P. aeruginosa, 1 isolate of C. freundii and 1 isolate of M. catarrhalis were tested for their efficacy in treatment of experimental mouse bacteremia of those isolates through dose-ranging and delayed-treatment studies. Efficacy of polyvalent phage preparations of different phages belonging to same species against pathogens of the same species was studied. Results showed that monovalent and polyvalent phage preparations were active in treatment of mice bacteremia after 45 min of injection of bacterial pathogens and on intervals of 5 h, 8 h, 14 h, 18 h and 24 h after bacterial pathogen injection.
Insights
Bacteriophages effectively treated experimental mouse bacteremia caused by common pathogens like Escherichia coli and Pseudomonas aeruginosa. Both single (monovalent) and mixed (polyvalent) phage preparations showed significant efficacy in treating bacterial infections in mice.
Area of Science:
- Microbiology
- Infectious Diseases
- Bacteriophage Therapy
Background:
- Neonatal septicemia poses a significant threat, often caused by common bacterial pathogens.
- Antibiotic resistance necessitates the exploration of alternative therapeutic strategies.
- Bacteriophages offer a promising alternative for combating bacterial infections.
Purpose of the Study:
- To determine the minimum lethal dose of five bacterial pathogens in mice.
- To evaluate the efficacy of bacteriophage preparations in treating experimental mouse bacteremia.
- To assess the effectiveness of both monovalent and polyvalent phage preparations against specific bacterial isolates.
Main Methods:
- Minimum lethal doses of Escherichia coli, Klebsiella pneumoniae, Haemophilus influenzae, Pseudomonas aeruginosa, Citrobacter freundii, and Moraxella catarrhalis were determined in mice.
- Bacteriophage preparations were tested against various isolates of these pathogens in dose-ranging and delayed-treatment studies.
- Efficacy was assessed in treating experimental mouse bacteremia following bacterial pathogen injection.
Main Results:
- Monovalent and polyvalent bacteriophage preparations demonstrated activity in treating mice bacteremia.
- Treatment efficacy was observed as early as 45 minutes post-bacterial injection.
- Effective treatment was achieved at various time intervals up to 24 hours after bacterial pathogen administration.
Conclusions:
- Bacteriophage therapy is a viable treatment option for experimental bacteremia caused by the studied pathogens.
- Both monovalent and polyvalent phage preparations show therapeutic potential.
- Phage therapy can be administered effectively even with delayed treatment intervals.
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