Biologically enhanced hydrogen sulfide absorption from sour gas under haloalkaline conditions

We studied a biotechnological desulfurization process for removal of toxic hydrogen sulfide (H₂S) from sour gas. The process consists of two steps: i) Selective absorption of H₂S into a (bi)carbonate solution in the absorber column and ii) conversion of sulfide to sulfur by sulfide oxidizing bacteria (SOB) in the aerated bioreactor. In previous studies, several physico-chemical factors were assessed to explain the observed enhancement of H₂S absorption in the absorber, but a full explanation was not provided. We investigated the relation between the metabolic activity of SOB and the enhancement factor. Two continuous experiments on pilot-scale were performed to determine H₂S absorption efficiencies at different temperatures and biomass concentrations. The absorption efficiency improved at increasing temperatures, i.e. H₂S concentration in the treated gas decreased from 715 ± 265 ppmv at 25.4 °C to 69 ± 25 ppmv at 39.4 °C. The opposite trend is expected when H₂S absorption is solely determined by physico-chemical factors. Furthermore, increasing biomass concentrations to the absorber also resulted in decreased H₂S concentrations in the treated gas, from approximately 6000 ppmv without biomass to 1664 ± 126 ppmv at 44 mg N/L. From our studies it can be concluded that SOB activity enhances H₂S absorption and leads to increased H₂S removal efficiencies in biotechnological gas desulfurization.