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Related Concept Videos

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Ribosome profiling or ribo-sequencing is a deep sequencing technique that produces a snapshot of active translation in a cell. It selectively sequences the mRNAs protected by ribosomes to get an insight into a cell’s translation landscape at any given point in time.
Applications of ribosome profiling
Ribosome profiling has many applications, including in vivo monitoring of translation inside a particular organ or tissue type and quantifying new protein synthesis levels.
The technique...
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Transcriptome Analysis of Single Cells
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Using single-cell transcriptomics to understand functional states and interactions in microbial eukaryotes.

Chuan Ku1, Arnau Sebé-Pedrós2,3

  • 1Institute of Plant and Microbial Biology, Academia Sinica, Taipei 11529, Taiwan.

Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences
|October 8, 2019
PubMed
Summary
This summary is machine-generated.

Single-cell transcriptomics offers a powerful lens into microbial eukaryotes, revealing their hidden life cycles and interactions. This technology illuminates protist biology and community dynamics, advancing our understanding of eukaryotic evolution.

Keywords:
environmental samplinggene expression analysisprotistsscRNA-sequnicellular eukaryotes

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Area of Science:

  • Microbiology
  • Eukaryotic Evolution
  • Genomics

Background:

  • Eukaryotic microorganism diversity and evolution present significant biological challenges.
  • Advances in discovery and phylogeny have reshaped the eukaryotic tree of life.
  • Knowledge gaps persist regarding microbial eukaryote life cycles, physiology, and interactions.

Purpose of the Study:

  • To explore the utility of high-throughput, genome-wide gene expression analysis in single eukaryotic cells.
  • To highlight the application of single-cell transcriptomics in understanding protist biology.
  • To discuss the potential of single-cell transcriptomics in microbial ecology.

Main Methods:

  • Review of single-cell transcriptomics methodologies, emphasizing microbial eukaryote applications.
  • Analysis of single-cell gene expression in cultured protists.
  • Envisioning applications for protist communities.

Main Results:

  • Single-cell transcriptomics provides insights into microbial eukaryote biology.
  • Methodologies are being refined for diverse applications.
  • Potential exists to analyze community composition and cellular states.

Conclusions:

  • Single-cell transcriptomics is crucial for understanding microbial eukaryote biology.
  • This approach can reveal cellular states and interspecific interactions within communities.
  • Further application of single-cell transcriptomics will significantly advance protist research.