Methane emissions of manure from dairy cows fed red clover- or corn silage-based diets supplemented with linseed oil

The objective of this study was to investigate the effects of forage source (red clover silage: RCS vs. corn silage: CS) and diet supplementation with linseed oil (LO) on CH₄ emissions of manure from dairy cows. For this purpose, 12 lactating cows were used in a 2 × 2 factorial arrangement of treatments. Cows were fed (ad libitum) RCS- or CS-based diets (forage:concentrate ratio 60:40; dry matter basis) without or with LO addition (4% dry matter). Feces and urine were collected from each cow and mixed with residual sludge obtained from a manure storage structure. Manure was incubated for 17 wk at 20°C under anaerobic conditions (O₂-free N₂) in 500-mL glass bottles. Methane emissions and changes in chemical composition of the manure were monitored during the entire incubation period. The total amount of feces and urine excreted by cows was not affected by dietary treatments and averaged 6.6 kg/d of volatile solids (VS). Compared with manure from cows fed RCS-based diets, maximum CH₄ production potential of manure from cows fed CS-based diets was 54% higher (182 vs. 118 L/kg of VS) throughout the incubation period. Maximum CH₄ production potential from manure also increased (by 17%) when cows were fed LO-supplemented diets compared with those fed nonsupplemented diets. Similar to maximum CH₄ production potential, VS degraded during incubation (i.e., VS loss) was higher from manure from cows fed CS-based diets versus cows fed RCS-based diets (30.6 vs. 22.5%), and increased (+3 percentage units, on average) with the addition of LO to the diets. Ammonia concentration in manure was higher when cows were fed CS-based diets compared with RCS-based diets, and declined with LO supplementation to CS and RCS diets. It is concluded that both dietary forage source and fat supplementation affect maximum CH₄ production potential from manure and this should be taken into account when such dietary options are recommended to mitigate enteric CH₄ emissions from dairy cows.