Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Induction of lambda prophage by furazolidone.

A K Pal, S N Chatterjee

    Mutation Research
    |April 1, 1985
    PubMed
    Summary

    Furazolidone induces prophages in a dose-dependent manner, with optimal induction occurring at 1.2 microgram/ml x h. Chloramphenicol inhibits this furazolidone-induced prophage induction, while higher furazolidone concentrations show toxicity.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    Precise oxygen therapy to emphysema patients by fuzzy-based gain tuning control of set-point regulated MRAC.

    Computers in biology and medicine·2026
    Same author

    Characterization of Forced Degradants of Tegafur, Gimeracil, and Oteracil Potassium by Liquid Chromatographic-Electrospray Ionization-Mass Spectrometry and Simultaneous Estimation of Triple Combination in Drug Substance and Finished Pharmaceutical Dosage Form.

    Archives of Razi Institute·2024
    Same author

    Clinical Significance of Faecal Calprotectin in Differentiating Inflammatory Bowel Disease from Irritable Bowel Syndrome.

    Mymensingh medical journal : MMJ·2024
    Same author

    Development and validation of a microwave-assisted digestion technique as a rapid sample preparation method for the estimation of selenium in pharmaceutical dosage forms by ICP-OES.

    Archives of Razi Institute·2024
    Same author

    Comment to "A comparative study between open pre-peritoneal approach versus laparoscopic trans-abdominal pre-peritoneal approach in recurrent inguinal hernia repair: a prospective cohort study".

    Hernia : the journal of hernias and abdominal wall surgery·2024
    Same author

    Oncological Clearance Following Resection of the Carcinoma Rectum: A Comparison between Laparoscopic and Open Anterior Resection.

    Mymensingh medical journal : MMJ·2024

    Area of Science:

    • Microbiology
    • Molecular Biology

    Background:

    • Prophage induction is a critical process in bacteriophage biology.
    • Understanding factors influencing prophage induction is essential for microbial genetics and phage therapy research.

    Purpose of the Study:

    • To investigate the dose-dependent effects of furazolidone on prophage induction.
    • To explore the influence of metabolic conditions and other agents on furazolidone-induced prophage activity.

    Main Methods:

    • Dose-response experiments with varying furazolidone concentrations.
    • Assays conducted with and without a metabolizing mixture.
    • Inhibition studies using chloramphenicol.
    • Analysis of burst size and latent period kinetics.

    Main Results:

    • Furazolidone induced prophages in a dose-dependent manner, peaking at 1.2 microgram/ml x h.
    • A metabolizing mixture enhanced furazolidone-induced prophage levels by 2-3 fold, reaching up to 70% efficiency.
    • Chloramphenicol inhibited furazolidone-induced prophage induction.
    • Higher furazolidone concentrations (>10 microgram/ml) were toxic, and burst size decreased with prolonged exposure.

    Conclusions:

    • Furazolidone is an effective agent for prophage induction, with its activity modulated by metabolic conditions.
    • The study elucidates the complex interaction between furazolidone, metabolic activity, and phage induction dynamics.
    • Chloramphenicol's inhibitory effect highlights specific molecular pathways involved in this process.

    Related Experiment Videos