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Updated: Jan 6, 2026

Bergmeyer Glucose Quantification for Microbiological Samples
Published on: January 17, 2025
Wavelength-ratiometric and colorimetric probes for glucose determination
Ramachandram Badugu1, Joseph R Lakowicz1, Chris D Geddes1,2
1Center for Fluorescence Spectroscopy, Department of Biochemistry and Molecular Biology, Medical Biotechnology Center, University of Maryland School of Medicine, 725 West Lombard St, Baltimore, MD 21201, USA.
Abstract:
We characterize two new water-soluble probes, m- and p-NBDPBA, which show notable changes in their absorption spectra in the presence of monosaccharides such as glucose and fructose. Subsequently glucose concentrations can readily be determined in the physiological glucose concentration range in a wavelength-ratiometric and colorimetric manner. m-NBDPBA has a dissociation constant of 204 mM for glucose, which is not unlike other mono-phenyl boronic acid derived probes. In addition we have also synthesized a control compound, NBDP, which does not contain the boronic acid moiety and is therefore insensitive to sugars, to help rationale the spectral changes observed.
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