Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Real Time RT-PCR

Real Time RT-PCR

Real-time reverse transcription-polymerase chain reaction, or Real-time RT-PCR, is an analytical tool used to determine the expression level of target genes. The method involves converting mRNA to complementary DNA with the help of an enzyme known as reverse transcriptase, followed by the PCR amplification of the cDNA. These two processes can be performed simultaneously in a single tube or separately as a two-step reaction.
The real-time quantification of the number of amplified products is...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Gardiquimod Nanoemulsion Targets Cutaneous Leishmaniasis Lesions Reducing Systemic Toxicity and Parasite Burden.

Advanced healthcare materials·2026

Same author

Adipose Tissue Plasticity and Adipogenesis: From Cellular Mechanisms to Therapeutic Targets in Obesity.

Current obesity reports·2026

Same author

Dual-Acting Vitamin B<sub>3</sub>-Melanostatin Neuropeptide Hybrids as Potent Modulators of the Dopamine D<sub>2</sub> Receptors with Neuroprotective Activity.

Journal of medicinal chemistry·2026

Same author

Repeated Oral Methylphenidate Administration Evokes Changes in Brain Plasticity Proteins in Juvenile Wistar Kyoto Rats: Evidence for Sex-Related Differences.

Neurochemical research·2026

Same author

Genetic Manipulation in <i>Sporothrix</i> Species: Molecular Tools, Challenges, and Applications.

Journal of fungi (Basel, Switzerland)·2026

Same author

Red Blood Cell Antioxidant State in Fanconi Anemia: The Highlighted Roles of Pi-Class Glutathione S-Transferase and Glutathione Peroxidase.

Antioxidants (Basel, Switzerland)·2025

Search research articles

Related Experiment Video

Updated: Jan 6, 2026

Real-time Analysis of Transcription Factor Binding, Transcription, Translation, and Turnover to Display Global Events During Cellular Activation

Real-time Analysis of Transcription Factor Binding, Transcription, Translation, and Turnover to Display Global Events During Cellular Activation

Published on: March 7, 2018

Methods for the analysis of transcriptome dynamics.

Daniela F Rodrigues¹, Vera M Costa¹, Ricardo Silvestre^2,3

¹UCIBIO , REQUIMTE , Laboratory of Toxicology , Faculty of Pharmacy , University of Porto , Rua Jorge Viterbo Ferreira , 228 , 4050-313 , Porto , Portugal . Email: danielacfrodrigues@gmail.com ;

Toxicology Research

|October 8, 2019

Summary

Understanding the transcriptome, the full set of RNA transcripts, is key to gene expression control. This review compares methods for analyzing RNA transcription and decay rates, crucial for understanding cellular responses.

More Related Videos

Determining Genome-wide Transcript Decay Rates in Proliferating and Quiescent Human Fibroblasts

Determining Genome-wide Transcript Decay Rates in Proliferating and Quiescent Human Fibroblasts

Published on: January 2, 2018

Measuring mRNA Levels Over Time During the Yeast S. cerevisiae Hypoxic Response

Measuring mRNA Levels Over Time During the Yeast S. cerevisiae Hypoxic Response

Published on: August 10, 2017

Related Experiment Videos

Last Updated: Jan 6, 2026

Real-time Analysis of Transcription Factor Binding, Transcription, Translation, and Turnover to Display Global Events During Cellular Activation

Real-time Analysis of Transcription Factor Binding, Transcription, Translation, and Turnover to Display Global Events During Cellular Activation

Published on: March 7, 2018

Determining Genome-wide Transcript Decay Rates in Proliferating and Quiescent Human Fibroblasts

Determining Genome-wide Transcript Decay Rates in Proliferating and Quiescent Human Fibroblasts

Published on: January 2, 2018

Measuring mRNA Levels Over Time During the Yeast S. cerevisiae Hypoxic Response

Measuring mRNA Levels Over Time During the Yeast S. cerevisiae Hypoxic Response

Published on: August 10, 2017

Area of Science:

Molecular Biology
Genomics
Biochemistry

Background:

The transcriptome encompasses all RNA types (rRNA, mRNA, tRNA, noncoding RNA) and its steady-state results from balanced transcription and decay rates.
Gene expression is primarily controlled at the transcription level, but RNA decay also plays a critical role through stabilization or degradation.
Transcriptome analysis reveals gene expression changes, offering insights into physiological and pathological pathways.

Purpose of the Study:

To critically compare methods for analyzing RNA transcription and decay contributions to gene expression dynamics.
To highlight the importance of understanding RNA synthesis and decay for deciphering gene expression regulatory mechanisms.
To provide an overview of data integration methods for transcriptome analysis.

Main Methods:

Comparative analysis of existing methodologies for quantifying RNA transcription rates.
Comparative analysis of existing methodologies for quantifying RNA decay rates.
Review of methods for integrating and analyzing transcriptome data.

Main Results:

The review critically evaluates various methods for assessing RNA transcription and decay.
It emphasizes the necessity of considering both RNA synthesis and degradation for a comprehensive understanding of gene expression.
The importance of data integration for system-level interpretation of transcriptome alterations is discussed.

Conclusions:

Accurate assessment of RNA transcription and decay is vital for understanding gene expression regulation and cellular responses.
Integrating transcriptome data provides a holistic view of gene expression dynamics and their implications.
This review aids researchers in selecting appropriate methods for transcriptome analysis, particularly in toxicology.