Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Defense Against Bacterial Pathogens01:31

Defense Against Bacterial Pathogens

2.6K
The human immune system is a complex network of cells, tissues, and organs that work together to defend the body against bacterial infections. It consists of various immune cells, each playing a specific role in the defense mechanism.
Phagocytes
Phagocytes are the frontline soldiers of the immune system. They include neutrophils and macrophages. Neutrophils are the most abundant type of white blood cell and are quickly mobilized to the site of infection. Macrophages are larger cells that patrol...
2.6K
Development of Antibiotic Resistance01:30

Development of Antibiotic Resistance

1.1K
Antibiotic resistance is a major public health concern that arises when bacteria evolve mechanisms to withstand the effects of antibiotic treatments. This resistance can be intrinsic, acquired through genetic mutations, or transferred between bacteria via horizontal gene transfer. The development of antibiotic resistance poses significant challenges in treating bacterial infections and necessitates ongoing research to develop new therapeutic strategies.Intrinsic resistance occurs when bacterial...
1.1K
Antibiotic Selection00:57

Antibiotic Selection

59.3K
Overview
59.3K
Combined Effects of Drugs: Synergism01:27

Combined Effects of Drugs: Synergism

6.7K
Synergism is a useful mechanism where combining two or more drugs is more effective than each constituent used alone. Such combinations are also called supra-additive interactions. The drugs collectively enhance the final therapeutic effect by acting on different targets. Another advantage is that the low dose of each constituent drug is sufficient to achieve the desired effect. This helps reduce the duration of therapy and lower the adverse effects of these drugs.
Such synergistic combinations...
6.7K
Biological Methods for Microbial Control01:28

Biological Methods for Microbial Control

748
Biological agents offer an effective means of controlling microbial growth by leveraging natural processes like predation, competition, and the secretion of antimicrobial substances.Predatory bacteria such as Bdellovibrio species target and kill pathogens like Salmonella and E. coli. They are widely used in poultry farms to control infections. Myxococcus species help combat plant-pathogenic fungi. These naturally occurring predators serve as eco-friendly alternatives to chemical pesticides and...
748
Antimicrobial Proteins01:23

Antimicrobial Proteins

12.9K
Antimicrobial proteins are important components of the immune system. They aid the body in combating pathogens by either killing them directly or hindering their replication processes. Four main types of antimicrobial substances are interferons, the complement system, iron-binding proteins, and antimicrobial proteins.
Interferons
Interferons (IFNs) are proteins produced by lymphocytes, macrophages, and fibroblasts infected with viruses. While IFNs cannot prevent viruses from entering and...
12.9K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

The hemodynamic effects of bolus norepinephrine and phenylephrine in the treatment of hypotensive events in preeclamptic women with severe features following spinal anesthesia during cesarean section: a randomized controlled trial.

Frontiers in pharmacology·2026
Same author

A case of primary prostatic intravascular large B-cell lymphoma misdiagnosed as benign prostatic hyperplasia.

Discover oncology·2026
Same author

Renal metastasis of adenocarcinoma of the gastrointestinal tract with unknown primary site: a case report and review of the literature.

Therapeutic advances in urology·2026
Same author

The inhibition mechanism of <i>Wolbachia</i> on mosquito-borne RNA virus replication as seen through lipid metabolism regulation.

Frontiers in microbiology·2026
Same author

Genomic Characterization of Antibiotic-Resistant <i>Enterococcus</i> Spp. Strains in Clinical Settings of Yichang, China.

Infection and drug resistance·2026
Same author

Upcycled Manganese Slag Enables Self-Regenerating Pyrrole-N Catalysis for Precision, Singlet Oxygen-Driven Antibiotic Detoxification by Peroxydisulfate Activation.

Small (Weinheim an der Bergstrasse, Germany)·2026

Related Experiment Video

Updated: Jan 6, 2026

Generating Transposon Insertion Libraries in Gram-Negative Bacteria for High-Throughput Sequencing
08:19

Generating Transposon Insertion Libraries in Gram-Negative Bacteria for High-Throughput Sequencing

Published on: July 7, 2020

11.2K

Targeting Bacterial Antioxidant Systems for Antibiotics Development.

Xiaoyuan Ren1, Lili Zou1,2, Arne Holmgren1

  • 1Division of Biochemistry, Department of Medical Biochemistry and Biophysics, Karolinska Institutet, SE-171 77 Stockholm, Sweden.

Current Medicinal Chemistry
|October 8, 2019
PubMed
Summary

Novel antibiotics targeting bacterial antioxidant systems show promise against multidrug-resistant bacteria. Inhibiting thioredoxin and glutathione pathways, like with ebselen and silver, offers a new strategy to combat resistant infections.

Keywords:
Multi-drug resistant bacteriaROSantibioticsebselenglutathione systemsilverthioredoxin system.

More Related Videos

Antibiotic Dereplication Using the Antibiotic Resistance Platform
10:49

Antibiotic Dereplication Using the Antibiotic Resistance Platform

Published on: October 17, 2019

11.5K
Stress-induced Antibiotic Susceptibility Testing on a Chip
12:41

Stress-induced Antibiotic Susceptibility Testing on a Chip

Published on: January 8, 2014

6.8K

Related Experiment Videos

Last Updated: Jan 6, 2026

Generating Transposon Insertion Libraries in Gram-Negative Bacteria for High-Throughput Sequencing
08:19

Generating Transposon Insertion Libraries in Gram-Negative Bacteria for High-Throughput Sequencing

Published on: July 7, 2020

11.2K
Antibiotic Dereplication Using the Antibiotic Resistance Platform
10:49

Antibiotic Dereplication Using the Antibiotic Resistance Platform

Published on: October 17, 2019

11.5K
Stress-induced Antibiotic Susceptibility Testing on a Chip
12:41

Stress-induced Antibiotic Susceptibility Testing on a Chip

Published on: January 8, 2014

6.8K

Area of Science:

  • Microbiology
  • Pharmacology
  • Biochemistry

Background:

  • Multidrug-resistant bacteria pose a significant threat to modern medicine, necessitating novel antibiotic development strategies.
  • Reactive Oxygen Species (ROS) are implicated in antibiotic-induced bacterial cell death, with antioxidant systems contributing to resistance.
  • Thioredoxin and glutathione systems are crucial thiol-dependent antioxidant pathways involved in essential bacterial processes.

Purpose of the Study:

  • To explore novel antibiotic development targeting bacterial antioxidant systems.
  • To investigate the potential of inhibiting thioredoxin and glutathione systems for combating bacterial infections.
  • To evaluate the efficacy of drug repurposing for novel antibiotic strategies.

Main Methods:

  • Investigated the role of Reactive Oxygen Species (ROS) in antibiotic action.
  • Examined the function of thioredoxin and glutathione systems in bacterial antioxidant defense and resistance.
  • Evaluated thioredoxin reductase inhibitors (ebselen, auranofin) against bacteria with varying antioxidant capacities.
  • Assessed the synergistic effect of combining ebselen with silver against Multidrug-Resistant (MDR) Gram-negative bacteria.

Main Results:

  • Antibiotic-induced bacterial cell death is mediated by Reactive Oxygen Species (ROS).
  • Thioredoxin and glutathione systems are key contributors to antibiotic resistance.
  • Ebselen and auranofin inhibit bacterial growth, particularly in strains lacking efficient glutathione systems.
  • A combination of ebselen and silver demonstrates significant synergistic activity against MDR Gram-negative bacteria.

Conclusions:

  • Targeting thiol-dependent antioxidant systems presents a viable strategy for developing new antibiotics.
  • Drug repurposing of ebselen and auranofin, especially in combination with agents like silver, shows promise for treating MDR bacterial infections.
  • These findings support the development of novel therapeutic approaches against challenging bacterial pathogens.