Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Conservative Site-specific Recombination and Phase Variation02:53

Conservative Site-specific Recombination and Phase Variation

6.6K
Because the DNA segments are cut and reorganized in a direction-specific manner, site-specific recombination has emerged as an efficient genetic engineering technique. Flippase and Cyclization recombinases or Flp and Cre, respectively, are two members of the tyrosine recombinase family derived from bacteriophages, that are used to mediate site-specific DNA insertions, deletions, and targeted expression of proteins in mammalian cell lines.
The recognition sites for Cre recombinase called LoxP...
6.6K
Plasmids01:28

Plasmids

986
Plasmids are extrachromosomal DNA molecules found in bacteria, archaea, and some eukaryotic microbes like yeast. These small, circular DNA structures typically contain fewer than 30 genes, although some may exist linearly. Plasmids vary in their number within a cell, known as copy number. Single-copy plasmids are present in one copy per cell and multi-copy plasmids are present in multiple copies, reaching over 100 copies per cell.Plasmids usually replicate independently of the chromosomal DNA...
986

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Author Correction: Magnetoreception in a freshwater ciliate arises from endosymbiosis.

Nature communications·2026
Same author

Magnetoreception in a freshwater ciliate arises from endosymbiosis.

Nature communications·2026
Same author

Sulfur disproportionation occurs globally across anoxic habitats and has multiple mechanisms of independent evolutionary origin.

The ISME journal·2026
Same author

CRISPR spacers reveal diverse and abundant Thermococcales viruses in hydrothermal vents.

Research square·2026
Same author

Extensive lateral gene transfer between proto-eukaryotes and <i>Heimdallarchaeia</i> suggests their close association during eukaryogenesis.

mLife·2025
Same author

Complete genome sequence of fluoroacetate-degrading <i>Caballeronia</i> sp. S22 strain (DSM 8341) as a reference resource for investigations of microbial defluorination.

Microbiology resource announcements·2024

Related Experiment Video

Updated: Jan 6, 2026

CAPRRESI: Chimera Assembly by Plasmid Recovery and Restriction Enzyme Site Insertion
07:37

CAPRRESI: Chimera Assembly by Plasmid Recovery and Restriction Enzyme Site Insertion

Published on: June 25, 2017

12.0K

WASPS: web-assisted symbolic plasmid synteny server.

Catherine Badel1, Violette Da Cunha1, Ryan Catchpole1

  • 1Microbiology Department, CEA, CNRS, Univ. Paris-Sud, Institute for Integrative Biology of the Cell (I2BC), Université Paris-Saclay, Gif-sur-Yvette cedex 91198, France.

Bioinformatics (Oxford, England)
|October 8, 2019
PubMed
Summary

Web-Assisted Symbolic Plasmid Synteny (WASPS) offers new tools for analyzing plasmid genomes across bacteria, archaea, and eukaryotes. This web service provides sequence similarity searches and real-time synteny map generation for comprehensive plasmid analysis.

More Related Videos

Identification of Plasmodesmal Localization Sequences in Proteins In Planta
08:07

Identification of Plasmodesmal Localization Sequences in Proteins In Planta

Published on: August 15, 2017

8.7K
Plasmid-derived DNA Strand Displacement Gates for Implementing Chemical Reaction Networks
07:50

Plasmid-derived DNA Strand Displacement Gates for Implementing Chemical Reaction Networks

Published on: November 25, 2015

14.8K

Related Experiment Videos

Last Updated: Jan 6, 2026

CAPRRESI: Chimera Assembly by Plasmid Recovery and Restriction Enzyme Site Insertion
07:37

CAPRRESI: Chimera Assembly by Plasmid Recovery and Restriction Enzyme Site Insertion

Published on: June 25, 2017

12.0K
Identification of Plasmodesmal Localization Sequences in Proteins In Planta
08:07

Identification of Plasmodesmal Localization Sequences in Proteins In Planta

Published on: August 15, 2017

8.7K
Plasmid-derived DNA Strand Displacement Gates for Implementing Chemical Reaction Networks
07:50

Plasmid-derived DNA Strand Displacement Gates for Implementing Chemical Reaction Networks

Published on: November 25, 2015

14.8K

Area of Science:

  • Genomics
  • Bioinformatics
  • Molecular Biology

Background:

  • Comparative plasmid genome analysis is challenging due to complex tools and limited databases.
  • Existing plasmid databases primarily focus on bacteria and offer only sequence similarity searches.

Purpose of the Study:

  • To develop a user-friendly web service for comprehensive plasmid genome analysis.
  • To provide tools for sequence similarity searches and synteny mapping across diverse plasmid origins.

Main Methods:

  • Developed Web-Assisted Symbolic Plasmid Synteny (WASPS), a web service.
  • Created a database of all completely sequenced natural plasmids (bacterial, archaeal, eukaryal).
  • Implemented pre-calculated orthologous protein clustering for real-time synteny map generation.

Main Results:

  • WASPS enables protein and DNA sequence similarity searches against a comprehensive plasmid database.
  • The service generates fully resolved plasmid synteny maps in real time.
  • WASPS supports all modern web browsers except Internet Explorer.

Conclusions:

  • WASPS simplifies comparative plasmid genome analysis for researchers.
  • The tool facilitates a deeper understanding of plasmid diversity and evolution across domains of life.