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Kinetic Screening of Nuclease Activity using Nucleic Acid Probes

Kinetic Screening of Nuclease Activity using Nucleic Acid Probes

Published on: November 1, 2019

R-BIND: An Interactive Database for Exploring and Developing RNA-Targeted Chemical Probes.

Brittany S Morgan¹, Bilva G Sanaba², Anita Donlic²

¹Life Sciences Institute , University of Michigan , 210 Washtenaw Avenue , Ann Arbor , Michigan 48109 , United States.

ACS Chemical Biology

|October 8, 2019

Summary

This summary is machine-generated.

Researchers can now discover novel RNA-targeted chemical probes using the R-BIND database and its associated online tools. This resource aids in designing small molecules for RNA targeting, accelerating drug discovery and biological research.

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Area of Science:

Medicinal Chemistry
Chemical Biology
Molecular Biology

Background:

Targeting RNA with small molecules offers significant therapeutic potential but faces challenges in achieving specific recognition.
Developing effective RNA-targeted chemical probes is crucial for advancing RNA biology and drug discovery.

Purpose of the Study:

To create and launch an accessible online platform featuring the RNA-targeted BIoactive ligaNd Database (R-BIND).
To provide researchers with curated data and user-friendly tools for the discovery and development of RNA-targeted chemical probes.

Main Methods:

Curated the R-BIND database, compiling information on non-rRNA-targeting chemical probes with reported biological activity.
Performed cheminformatics analysis to identify RNA-privileged properties and validated these findings on an expanded dataset.
Developed an online platform with search functionalities, probe development tools, and a nearest neighbor algorithm for molecule similarity assessment.

Main Results:

The R-BIND database was updated and made publicly available through an online platform.
Cheminformatics analysis confirmed that key physicochemical, structural, and spatial properties of RNA-targeted ligands are conserved despite database expansion.
User-friendly tools were implemented for querying by various parameters and assessing molecule similarity to known ligands.

Conclusions:

The R-BIND database and associated online tools provide a valuable resource for the RNA-targeting small molecule research community.
This platform is expected to significantly aid researchers in designing novel chemical probes, optimizing lead compounds, and advancing the study of RNA functions.