Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Virtual reality based attentional bias modification training for patients with obsessive-compulsive disorder: a feasibility study.

Frontiers in psychiatry·2026
Same author

Research on single optical path difference crossfading technology for a coherent-dispersion spectrometer.

Optics express·2026
Same author

Measuring Cognitive Flexibility in Chinese Individuals with Obsessive-Compulsive Disorder: Psychometric Validation and Factor Structure of the Chinese Version of the Flexibility in Daily Life Scale.

Psychology research and behavior management·2026
Same author

Attentional bias abnormalities and their association with cognitive load in obsessive-compulsive disorder.

BMC psychiatry·2026
Same author

OptoRibo-seq for spatiotemporally resolved mapping of the local protein translatome.

Proceedings of the National Academy of Sciences of the United States of America·2026
Same author

GEfetch2R: fetching single-cell/bulk RNA-seq data from public repositories to R and benchmarking the subsequent format conversion tools.

GigaScience·2026

Related Experiment Video

Updated: Jan 6, 2026

Spatial Profiling of Protein and RNA Expression in Tissue: An Approach to Fine-Tune Virtual Microdissection
09:19

Spatial Profiling of Protein and RNA Expression in Tissue: An Approach to Fine-Tune Virtual Microdissection

Published on: July 6, 2022

5.3K

Mapping spatial transcriptome with light-activated proximity-dependent RNA labeling.

Pengchong Wang1,2,3,4,5, Wei Tang6,7, Zeyao Li1,8

  • 1School of Life Sciences, Tsinghua University, Beijing, China.

Nature Chemical Biology
|October 9, 2019
PubMed
Summary
This summary is machine-generated.

Researchers developed a new method, proximity-dependent photo-oxidation of RNA nucleobases (CAP-seq), to map RNA locations in live cells. This technique revealed specific messenger RNAs enriched at mitochondria, advancing spatial transcriptome studies.

More Related Videos

Method for Labeling Transcripts in Individual Escherichia coli Cells for Single-molecule Fluorescence In Situ Hybridization Experiments
07:51

Method for Labeling Transcripts in Individual Escherichia coli Cells for Single-molecule Fluorescence In Situ Hybridization Experiments

Published on: December 21, 2017

8.6K
Mapping RNA-RNA Interactions Globally Using Biotinylated Psoralen
11:32

Mapping RNA-RNA Interactions Globally Using Biotinylated Psoralen

Published on: May 24, 2017

12.5K

Related Experiment Videos

Last Updated: Jan 6, 2026

Spatial Profiling of Protein and RNA Expression in Tissue: An Approach to Fine-Tune Virtual Microdissection
09:19

Spatial Profiling of Protein and RNA Expression in Tissue: An Approach to Fine-Tune Virtual Microdissection

Published on: July 6, 2022

5.3K
Method for Labeling Transcripts in Individual Escherichia coli Cells for Single-molecule Fluorescence In Situ Hybridization Experiments
07:51

Method for Labeling Transcripts in Individual Escherichia coli Cells for Single-molecule Fluorescence In Situ Hybridization Experiments

Published on: December 21, 2017

8.6K
Mapping RNA-RNA Interactions Globally Using Biotinylated Psoralen
11:32

Mapping RNA-RNA Interactions Globally Using Biotinylated Psoralen

Published on: May 24, 2017

12.5K

Area of Science:

  • Molecular Biology
  • Cell Biology
  • Chemical Biology

Background:

  • RNA localization is crucial for eukaryotic cell function.
  • Existing tools for studying RNA spatial organization are limited.
  • Understanding the spatial transcriptome requires novel analytical methods.

Purpose of the Study:

  • To develop a chemical biology approach for spatially specific RNA labeling in live cells.
  • To investigate the local transcriptome near subcellular compartments.
  • To identify novel RNA localization patterns and their functional implications.

Main Methods:

  • Developed Chemical-activated, proximity-dependent photo-oxidation of RNA nucleobases (CAP-seq).
  • Utilized light-activated, proximity-dependent photo-oxidation for RNA labeling.
  • Employed affinity purification and high-throughput sequencing to identify labeled RNAs.

Main Results:

  • Demonstrated high spatial specificity in labeling RNA molecules within live cells.
  • Investigated local transcriptomes proximal to the endoplasmic reticulum and mitochondria.
  • Discovered enrichment of specific mRNAs (ribosomal proteins, oxidative phosphorylation) at the outer mitochondrial membrane.

Conclusions:

  • CAP-seq is a versatile and user-friendly technique for spatial transcriptome analysis.
  • The study provides new insights into RNA localization at the outer mitochondrial membrane.
  • This method has broad applicability for studying RNA organization in diverse biological systems.