Sapientia aquatica gen. nov., sp. nov., isolated from a crater lake

A new aerobic betaproteobacterium, strain SA-152^T, was isolated from the water of a crater lake. 16S rRNA gene sequence analysis revealed that strain SA-152^T belonged to the family Oxalobacteraceae (order Burkholderiales) and was phylogenetically related to Solimicrobium silvestre S20-91^T with 97.09 % and to Herminiimonas arsenicoxydans ULPAs1^T with 96.00 % 16S rRNA gene pairwise sequence similarity. Cells of strain SA-152^T were rod-shaped, non-motile, oxidase-negative and catalase-positive. Its fatty acid profile was dominated by two fatty acids, C_16 : 1 ω7c and C_16 : 0, the major respiratory quinones were Q-8 and Q-7, and the main polar lipids were phosphatidylethanolamine, phosphatidylglycerol and diphosphatidylglycerol. The G+C content of the genomic DNA of strain SA-152^T was 48.3 mol%. The new bacterium can be distinguished from closely related genera Solimicrobium, Herminiimonas, Rugamonas and Undibacterium based on its non-motile and oxidase-negative cells. On the basis of the phenotypic, chemotaxonomic and genomic data, strain SA-152^T is considered to represent a novel species of a new genus, for which the name Sapientia aquatica gen. nov., sp. nov. is proposed. The type strain of Sapientia aquatica is SA-152^T (=DSM 29805^T=NCAIM B.02613^T).