Marinobacter vulgaris sp. nov., a moderately halophilic bacterium isolated from a marine solar saltern

A facultatively anaerobic, Gram-stain-negative and non-gliding bacterium, designated F01^T, was isolated from marine solar saltern in Weihai, PR China. Cells of F01^T were 0.2-0.4 µm wide and 1.4-4.1 µm long, weakly catalase-positive and oxidase-negative. Growth of F01^T was determined to occur at 4-40 °C (optimum, 33-37 °C), pH 6.5-8.5 (optimum, 7.0-8.0), and with 0.5-18.0 % (w/v) NaCl (optimum, 3.0-6.0 %). The 16S rRNA gene sequence analysis indicated that F01^T represented a member of the genus Marinobacter within the family Alteromonadaceae. Phylogenetic analysis based on 16S rRNA gene sequences revealed that the isolate was most closely related to Marinobacter algicola DSM 16394^T, with a sequence similarity of 97.5 %. The DNA G+C content of the isolate was 57.6 mol%. The major respiratory quinone of F01^T was ubiquinone-9 (Q-9) and the major fatty acids were anteiso-C_15 : 0, C_16 : 0 and C_18 : 1ω9c. The major polar lipids were phosphoaminolipid, phosphatidylglycerol and phosphatidylethanolamine. On the basis of the results of the phylogenetic analysis and phenotypic properties, it is concluded that F01^T can be considered to represent a novel species in the genus Marinobacter, for which the name Marinobacter vulgaris sp. nov. is proposed. The type strain is F01^T (=MCCC 1H00290^T=KCTC 52700^T).