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Updated: Jan 6, 2026

Subtyping of Campylobacter jejuni ssp. doylei Isolates Using Mass Spectrometry-based PhyloProteomics MSPP
Published on: October 30, 2016
Analysis of Escherichia coli and Listeria monocytogenes Isolate Subtyping and Reporting Capacity by Public Health
DeAndrea Martinez1, Tanya Telfair LeBlanc, Kelley B Hise
1Center for Preparedness and Response (Ms Martinez and Dr LeBlanc) and National Center for Emerging and Zoonotic Infectious Diseases (Ms Hise), Centers for Disease Control and Prevention, Atlanta, Georgia.
Objectives:
To demonstrate the performance of 53 laboratories required to submit 90% or more of their pulsed-field gel electrophoresis (PFGE) subtyping results for Escherichia coli O157:H7 (E coli O157:H7) and Listeria monocytogenes (L monocytogenes) to the PulseNet national databases at the Centers for Disease Control and Prevention (CDC) within 4 working days of receiving isolates.
Methods:
We examined data from 53 laboratories during 2013-2017 to ascertain whether E. coli O157:H7 and L monocytogenes PFGE data were reported to the PulseNet national databases within 4 working days.
Results:
In the study period, 45 laboratories that submitted reports during the period (86.8%) met the target for timely submission of 10 606 (94.85%) E coli O157:H7 isolates into the PulseNet national database. For L monocytogenes isolates, 32 laboratories submitted reports (76.95%) that achieved timely submission of 3119 (93.35%) isolates.
Conclusions:
This study uncovered areas for improvement to advance public health in the CDC-funded laboratories.
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