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DNA Isolation

DNA Isolation

DNA isolation protocols can be fast and straightforward or complex and time-consuming depending on the type and quality of DNA required for further processing. For example, plasmid DNA extraction is a bit more complicated than genomic DNA extraction because of the need for an appropriate lysis method to separate plasmid DNA from gDNA during isolation. However, for specific applications, such as long-range DNA sequencing that require a good yield of high- quality DNA samples, we need to follow...

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Updated: Jan 6, 2026

Filtration Isolation of Nucleic Acids: A Simple and Rapid DNA Extraction Method

Filtration Isolation of Nucleic Acids: A Simple and Rapid DNA Extraction Method

Published on: August 6, 2016

A simple blood preparation method for nucleic acid amplification tests using membranes.

Jong Kun Kim¹, Young Mi Lee², Seon Duk Lee²

¹Department of Emergency Medicine, School of Medicine, Kyungpook National University, Daegu, Korea.

Technology and Health Care : Official Journal of the European Society for Engineering and Medicine

|October 10, 2019

Summary

A new method uses two membranes to separate serum from whole blood without electricity or instruments. This allows for pathogen detection using polymerase chain reaction (PCR) even in remote areas.

Keywords:

Membrane blood centrifugation nucleic acid amplification

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Last Updated: Jan 6, 2026

Filtration Isolation of Nucleic Acids: A Simple and Rapid DNA Extraction Method

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Published on: August 6, 2016

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Published on: August 6, 2011

Area of Science:

Biotechnology
Molecular Diagnostics
Point-of-Care Testing

Background:

Serum separation from whole blood is crucial for pathogen detection via nucleic acid amplification.
Centrifugation, the standard method, is impractical in electricity-limited rural settings.

Purpose of the Study:

To develop an instrument-free method for preparing serum suitable for nucleic acid amplification.
To enable bloodborne pathogen detection in resource-limited environments.

Main Methods:

Utilized two distinct membrane sheets to separate serum from the cellular fraction of whole blood.
Applied brief heating to the separated serum for DNA extraction.
Performed polymerase chain reaction (PCR) to amplify bacterial DNA from the processed serum.

Main Results:

Successfully separated serum from cellular components using the membrane filtration technique.
Obtained sufficient DNA quality and concentration from heated serum for effective PCR.
Observed amplified gene products, confirming the presence of Escherichia coli (E. coli).

Conclusions:

Demonstrated the feasibility of separating bacteria-containing serum using a simple two-membrane system.
Confirmed that DNA isolated from the serum processed by this method is suitable for PCR analysis after heating.
This technique offers a viable solution for molecular diagnostics in settings lacking electricity and specialized equipment.