Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

¹³C NMR: Distortionless Enhancement by Polarization Transfer (DEPT)01:20

¹³C NMR: Distortionless Enhancement by Polarization Transfer (DEPT)

1.6K
When proton-coupled carbon-13 spectra are simplified by a broadband proton decoupling technique, structural information about the coupled protons is lost. Distortionless enhancement by polarization transfer (DEPT) is a technique that provides information on the number of hydrogens attached to each carbon in a molecule. While the DEPT experiment utilizes complex pulse sequences, the pulse delay and flip angle are specifically manipulated. The resulting signals have different phases depending on...
1.6K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Combination of single-molecule Förster resonance energy transfer and hydrogen-deuterium exchange mass spectrometry toward dynamic structural biology.

Current opinion in structural biology·2026
Same author

An allosteric network governs Tom70 conformational dynamics to coordinate mitochondrial import.

Structure (London, England : 1993)·2025
Same author

Synthetic Type III-E CRISPR-Cas Effectors for Programmable RNA-targeting.

Journal of molecular biology·2025
Same author

The Mechanism of Histone Ubiquitylation by the ASB9-CUL5 Ubiquitin Ligase.

Molecular & cellular proteomics : MCP·2025
Same author

A Structurally Divergent Class Ia Ribonucleotide Reductase from a Tick-Borne Pathogen.

Biochemistry·2025
Same author

An allosteric network governs Tom70 conformational dynamics to coordinate mitochondrial protein import.

bioRxiv : the preprint server for biology·2025

Related Experiment Video

Updated: Jan 6, 2026

A Hydrogen-Deuterium Exchange Mass Spectrometry HDX-MS Platform for Investigating Peptide Biosynthetic Enzymes
11:32

A Hydrogen-Deuterium Exchange Mass Spectrometry HDX-MS Platform for Investigating Peptide Biosynthetic Enzymes

Published on: May 4, 2020

8.6K

DECA, A Comprehensive, Automatic Post-processing Program for HDX-MS Data.

Ryan J Lumpkin1, Elizabeth A Komives1

  • 1Department of Chemistry and Biochemistry, University of California, San Diego, 9500 Gilman Drive, La Jolla, CA 92092-0378.

Molecular & Cellular Proteomics : MCP
|October 10, 2019
PubMed
Summary

A new software package, Deuterium Exchange Correction and Analysis (DECA), offers comprehensive downstream processing for amide hydrogen/deuterium exchange mass spectrometry (HDX-MS) data. This tool aids in analyzing protein-ligand interactions and protein dynamics with improved accuracy and data visualization.

Keywords:
Biophysical methodsHDX-MScomputer modelingdata evaluationmacromolecular complex analysismolecular dynamicsprotein structurestatistics

More Related Videos

Millisecond Hydrogen/Deuterium-Exchange Mass Spectrometry for the Study of Alpha-Synuclein Structural Dynamics Under Physiological Conditions
08:40

Millisecond Hydrogen/Deuterium-Exchange Mass Spectrometry for the Study of Alpha-Synuclein Structural Dynamics Under Physiological Conditions

Published on: June 23, 2022

3.4K
Capillary Electrophoresis-based Hydrogen/Deuterium Exchange for Conformational Characterization of Proteins with Top-down Mass Spectrometry
05:45

Capillary Electrophoresis-based Hydrogen/Deuterium Exchange for Conformational Characterization of Proteins with Top-down Mass Spectrometry

Published on: June 8, 2021

3.7K

Related Experiment Videos

Last Updated: Jan 6, 2026

A Hydrogen-Deuterium Exchange Mass Spectrometry HDX-MS Platform for Investigating Peptide Biosynthetic Enzymes
11:32

A Hydrogen-Deuterium Exchange Mass Spectrometry HDX-MS Platform for Investigating Peptide Biosynthetic Enzymes

Published on: May 4, 2020

8.6K
Millisecond Hydrogen/Deuterium-Exchange Mass Spectrometry for the Study of Alpha-Synuclein Structural Dynamics Under Physiological Conditions
08:40

Millisecond Hydrogen/Deuterium-Exchange Mass Spectrometry for the Study of Alpha-Synuclein Structural Dynamics Under Physiological Conditions

Published on: June 23, 2022

3.4K
Capillary Electrophoresis-based Hydrogen/Deuterium Exchange for Conformational Characterization of Proteins with Top-down Mass Spectrometry
05:45

Capillary Electrophoresis-based Hydrogen/Deuterium Exchange for Conformational Characterization of Proteins with Top-down Mass Spectrometry

Published on: June 8, 2021

3.7K

Area of Science:

  • Biochemistry
  • Structural Biology
  • Proteomics

Background:

  • Amide hydrogen-deuterium exchange mass spectrometry (HDX-MS) is crucial for studying protein interactions and dynamics.
  • Existing HDX-MS platforms offer limited downstream processing capabilities.
  • A need exists for integrated software to handle complex HDX-MS data analysis.

Purpose of the Study:

  • To introduce the Deuterium Exchange Correction and Analysis (DECA) software package.
  • To provide a comprehensive solution for downstream processing of HDX-MS data.
  • To demonstrate the utility of DECA for analyzing protein-ligand interfaces, protein-protein interactions, and dynamic allostery.

Main Methods:

  • Development of the DECA software package.
  • Implementation of automatic back-exchange correction.
  • Inclusion of tools for overlapping peptide analysis and relative deuterium uptake calculations.
  • Integration of rigorous statistical analysis and high-quality figure generation.
  • Demonstration of DECA's functions using sample HDX-MS data.

Main Results:

  • DECA provides a comprehensive suite of downstream processing tools for HDX-MS data.
  • The software enables accurate analysis of deuterium uptake, even with overlapping peptides.
  • DECA facilitates robust statistical assessment of uptake differences.
  • High-quality figures for data presentation are generated by the software.

Conclusions:

  • DECA addresses the need for a comprehensive software solution for HDX-MS data analysis.
  • The software enhances the ability to map protein interfaces and understand protein dynamics.
  • DECA is a valuable tool for researchers utilizing amide hydrogen/deuterium exchange mass spectrometry.