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miR-26 suppresses renal cell cancer via down-regulating coronin-3

Xin Jun Wang1,2, Zhi Jian Yan1,2, Guang Cheng Luo3,4

  • 1Department of Urology, Zhongshan Hospital Xiamen University, Xiamen, 361000, Fujian, China.

Molecular and Cellular Biochemistry
|October 10, 2019
PubMed

Insights

Coronin-3 protein promotes renal cell cancer progression and metastasis. MicroRNA-26 (miR-26) targets coronin-3, inhibiting cancer cell growth and invasion, suggesting potential therapeutic strategies.

Area of Science:

  • Oncology
  • Molecular Biology
  • Biochemistry

Background:

  • Coronin-3 (coronin-1C) is an F-actin-binding protein implicated in metastasis in various cancers.
  • Its role in renal cell cancer (RCC) has not been previously investigated.

Purpose of the Study:

  • To investigate the expression and function of coronin-3 in renal cell cancer.
  • To elucidate the regulatory relationship between miR-26 and coronin-3 in RCC.

Main Methods:

  • Western blot assay and dual-luciferase reporter system to confirm miR-26 targeting of coronin-3.
  • MTT and colony formation assays for cell proliferation.
  • Wound healing and Transwell assays for cell migration and invasion.

Main Results:

  • miR-26 directly targets and down-regulates coronin-3 expression in renal cancer cells.
  • miR-26 significantly suppresses renal cancer cell proliferation, migration, and invasion.
  • Overexpression of coronin-3 reverses the inhibitory effects of miR-26 on cell growth and metastasis.

Conclusions:

  • Coronin-3 plays a crucial role in promoting renal cell cancer progression and metastasis.
  • miR-26 acts as a tumor suppressor by targeting coronin-3.
  • Coronin-3 represents a potential therapeutic target for renal cell cancer, and miR-26 offers a candidate for miRNA-based therapy.
Keywords:
Coronin-3InvasionMigrationProliferationRenal cell cancermiR-26

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