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Detection and Quantification of Nitrifying Bacteria Using Real-Time PCR.

Engil I P Pereira¹, Marcelo C M Teixeira Filho²

¹School of Earth, Environmental, and Marine Sciences, The University of Texas Rio Grande Valley, Edinburg, TX, USA.

Methods in Molecular Biology (Clifton, N.J.)

|October 10, 2019

Summary

This study details a method for quantifying soil nitrifying bacteria using real-time quantitative polymerase chain reaction (PCR). This technique helps understand nitrogen cycling and potential environmental impacts from nitrate pollution.

Keywords:

Amplification efficiency DNA extraction Nitrification Nitrifying gene Nitrosomonas europaea

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Area of Science:

Environmental microbiology
Soil science
Molecular biology

Background:

Nitrification, the conversion of ammonium to nitrate, is crucial for plant nitrogen uptake.
Nitrate mobility in soil poses environmental pollution risks.
Estimating nitrifying bacteria abundance complements process-level nitrification studies.

Purpose of the Study:

To present a detailed protocol for detecting and quantifying nitrifying bacteria in soil.
To enable accurate assessment of microbial communities involved in nitrogen cycling.

Main Methods:

Real-time quantitative polymerase chain reaction (qPCR) applied to soil samples.
Molecular techniques for bacterial detection and quantification.

Main Results:

Established a reliable qPCR procedure for soil nitrifying bacteria.
Quantified nitrifying bacteria populations in soil samples.

Conclusions:

qPCR provides a robust method for assessing nitrifying bacteria abundance.
This technique aids in managing soil nitrogen and mitigating environmental pollution.