Related Experiment Video

Updated: Jan 6, 2026

Spatiotemporal Analysis of Cytokinetic Events in Fission Yeast
11:19

Spatiotemporal Analysis of Cytokinetic Events in Fission Yeast

Published on: February 20, 2017

7.6K

Spindle pole body duplication defective yeast cells are more prone to membrane damage

Hatice Büşra Konuk1, Bengü Ergüden1

  • 1Department of Bioengineering, Gebze Technical University, Gebze 41400, Kocaeli, Turkey.

Mycologia
|October 10, 2019
PubMed

Insights

Yeast cells with defective spindle pole body (SPB) duplication and active SESA complex show increased membrane susceptibility to essential oils, impacting cell integrity.

Area of Science:

  • Cell Biology
  • Molecular Biology
  • Mycology

Background:

  • Chromosome segregation during mitosis is crucial for preventing genetic instability.
  • Spindle pole body (SPB) duplication is essential for accurate chromosome separation.
  • The MonoPolar Spindle 2 (MPS2) protein is vital for SPB insertion into the nuclear envelope.

Purpose of the Study:

  • To investigate the impact of essential oils on yeast cell membranes.
  • To explore the relationship between the SESA complex, SPB duplication, and membrane integrity.
  • To understand how genetic defects in SPB duplication affect susceptibility to essential oils.

Main Methods:

  • Utilizing yeast strains with defective SPB duplication (mps2∆ 2µm-SMY2 and mps2∆ pom34∆).
  • Activating the SESA complex in these yeast strains.
  • Treating yeast cells with various essential oils.
  • Assessing yeast cell membrane damage post-treatment.

Main Results:

  • Yeast cells with active SESA complex and defective SPB duplication exhibit heightened sensitivity to essential oil-induced membrane damage.
  • The SESA complex, known to influence membrane lipid composition, plays a role in this increased susceptibility.
  • Essential oils compromise the membrane integrity of these genetically modified yeast cells.

Conclusions:

  • The SESA complex and SPB duplication status significantly influence yeast cell membrane's response to essential oils.
  • Essential oils pose a greater risk to yeast cells with compromised SPB duplication mechanisms.
  • This study links genetic factors affecting cell division to susceptibility to external membrane-disrupting agents.
Keywords:
Essential oilMps2Saccharomyces cerevisiaemembrane damagespindle pole body duplication

More Related Videos

Examination of Mitotic and Meiotic Fission Yeast Nuclear Dynamics by Fluorescence Live-cell Microscopy12:04

Examination of Mitotic and Meiotic Fission Yeast Nuclear Dynamics by Fluorescence Live-cell Microscopy

Published on: June 24, 2019

10.5K
Manipulation and Analysis of Cell Cycle-Dependent Processes in Budding Yeast08:13

Manipulation and Analysis of Cell Cycle-Dependent Processes in Budding Yeast

Published on: September 26, 2025

419

Related Concept Videos

Centrosome Duplication02:25

Centrosome Duplication

The primary microtubule organizing center (MTOC) in animal cells is the centrosome. A centrosome has two cylindrical centrioles at its core. Each centriole consists of nine sets of three microtubules held together by proteins. The centrioles are positioned at right angles to each other and surrounded by a shapeless protein cloud called the pericentriolar matrix, or pericentriolar material (PCM).
To ensure that each daughter cell receives a centrosome after cell division, centrosome duplication...
4.7K
Nondisjunction01:29

Nondisjunction

During meiosis, chromosomes occasionally separate improperly. This occurs due to failure of homologous chromosome separation during meiosis I or failed sister chromatid separation during meiosis II. In some species, notably plants, nondisjunction can result in an organism with an entire additional set of chromosomes, which is called polyploidy. In humans, nondisjunction can occur during male or female gametogenesis and the resulting gametes possess one too many or one too few chromosomes.
81.6K
Nondisjunction01:21

Nondisjunction

Nondisjunction is the failure of homologous chromosomes or sister chromatids to separate correctly and move to the opposite poles of the cells. This produces daughter cells with abnormal chromosome numbers.  Nondisjunction is common during anaphase I or anaphase II of meiosis.  Mutations in synaptonemal complex proteins that attach homologous chromosomes increase the chances of nondisjunction in anaphase I of meiosis I. In contrast, mutations in topoisomerases and condensins that hold...
4.7K
Meiosis vs. Mitosis02:57

Meiosis vs. Mitosis

Cell division is necessary for growth and reproduction in organisms. Mitosis aids cell growth and development by dividing somatic cells. In contrast, meiosis causes the division of germ cells and plays an essential role in sexual reproduction. Due to their unique functional requirements, mitosis and meiosis differ from each other in multiple aspects.
Before the start of mitosis and meiosis I, the cell synthesizes DNA, resulting in two homologous copies of each chromosome. DNA synthesis is...
69.1K
The Spindle Assembly Checkpoint02:19

The Spindle Assembly Checkpoint

The spindle assembly checkpoint is a molecular surveillance mechanism ensuring the fidelity of chromosome segregation during anaphase. The checkpoint monitors the completion of all the prerequisite steps before chromosome segregation to determine whether the segregation process should proceed or be delayed.
Many proteins function together to control the spindle assembly checkpoint. Mutations affecting these proteins may allow cells to proceed into anaphase prematurely, resulting in the...
3.6K