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Related Experiment Videos

Neurospora trehalase and its structural gene.

C White, D B Lee, S J Free

    Genetics
    |June 1, 1985
    PubMed
    Summary

    Neurospora mutants lacking trehalase enzyme activity were identified. These mutations impact enzyme stability and lead to the production of an inhibitor, likely the mutant trehalase itself, affecting wild-type enzyme function.

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    Area of Science:

    • Biochemistry
    • Molecular Biology
    • Genetics

    Background:

    • Trehalase is a key enzyme involved in carbohydrate metabolism.
    • Understanding trehalase function and regulation is crucial for metabolic studies.

    Purpose of the Study:

    • To isolate and characterize Neurospora mutants with altered trehalase activity.
    • To investigate the molecular basis of trehalase inhibition and regulation.

    Main Methods:

    • Isolation and genetic mapping of Neurospora trehalaseless mutants.
    • Purification and characterization of wild-type trehalase.
    • Enzyme activity assays and inhibitor studies.

    Main Results:

    • The trehalase structural gene was mapped to linkage group I.
    • Mutations affected enzyme thermostability and led to the production of a trehalase inhibitor.
    • Wild-type trehalase was purified as a tetramer (88,000 MW subunits).
    • Trehalase production is glucose-repressible, with induction occurring 60 minutes after glucose removal.

    Conclusions:

    • Mutant trehalase subunits may act as inhibitors by forming non-functional heteromultimers with wild-type subunits.
    • Trehalase expression in Neurospora is tightly regulated by glucose availability.

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