Treatment of feces from beef cattle fed the enteric methane inhibitor 3-nitrooxypropanol

The study evaluated the residual effect of the known enteric methane inhibitor 3-nitrooxypropanol (3NOP) on anaerobic digestion of cattle feces (feces) in a CH₄ potential batch test and two consecutive runs of an anaerobic leach bed reactor at a solids retention time of 40 days. The feces used in this study were collected from beef cattle fed forage- (backgrounding) or grain- (finishing) based diets supplemented with 3NOP in feedlot and metabolism studies. The results showed that CH₄ yields were not significantly different from treatments using control feces and feces collected from cattle fed a diet supplemented with 3NOP in both CH₄ potential and leach bed studies. Spiking feces with 200 mg 3NOP kg^-1 dry matter decreased CH₄ production rate by 8.0-18.1% estimated from the Gompertz equation, increased the lag phase time (0.4-3.4 d) in all the treatments, while there was no significant difference in the overall CH₄ yield. Results from this study showed that 3NOP can be used as an effective enteric CH₄ inhibitor with no residual effect on anaerobic digestion.