Oxygen Regulates Nitrous Oxide Production Directly in Agricultural Soils

Oxygen (O₂) plays a critical and yet poorly understood role in regulating nitrous oxide (N₂O) production in well-structured agricultural soils. We investigated the effects of in situ O₂ dynamics on N₂O production in a typical intensively managed Chinese cropping system under a range of environmental conditions (temperature, moisture, ammonium, nitrate, dissolved organic carbon, and so forth). Climate and management (fertilization, irrigation, precipitation, and temperature), and their interactions significantly affected soil O₂ and N₂O concentrations (P < 0.05). Soil O₂ concentration was the most significant factor correlating with soil N₂O concentration (r = -0.71) when compared with temperature, water-filled pore space, and ammonium concentration (r = 0.30, 0.25, and 0.26, respectively). Soil N₂O concentration increased exponentially with decreasing soil O₂ concentrations. The exponential model of N treatments and fertilization with irrigation/precipitation events predicted 74-90% and 58% of the variance in soil N₂O concentrations, respectively. Our results highlight that the soil O₂ status is the proximal, direct, and the most decisive environmental trigger for N₂O production, outweighing the effects of other factors and could be a key variable integrating the aggregated effects of various complex interacting variables. This study offers new opportunities for developing more sensitive approaches to predicting and through appropriate management interventions mitigating N₂O emissions from agricultural soils.