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Lymphocyte membrane alterations caused by nylon wool column separation.

A Corrigan, R O'Kennedy, H Smyth

    Journal of Immunological Methods
    |January 1, 1979
    PubMed
    Summary
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    The nylon wool column method depletes vital cell membrane components in lymphocytes, impacting T cells. This makes the method unsuitable for biochemical analysis of these immune cells.

    Area of Science:

    • Immunology
    • Cell Biology
    • Biochemistry

    Background:

    • Lymphocyte separation is crucial for immunological studies.
    • The nylon wool column method is a common technique for isolating T lymphocytes.
    • Previous research has not fully characterized the biochemical impact of this method on lymphocytes.

    Purpose of the Study:

    • To assess the biochemical integrity of lymphocytes after separation using the nylon wool column method.
    • To identify specific cellular components affected by this separation technique.
    • To determine the suitability of the nylon wool column method for biochemical analyses.

    Main Methods:

    • Lymphocytes were separated using the nylon wool column method.
    • Cell viability was assessed post-separation.

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  • Cellular levels of phospholipid, cholesterol, and sialic acid were quantified.

    • Main Results:

    • Lymphocyte preparations remained viable after nylon wool column separation.
    • Significant depletion of phospholipid, cholesterol, and sialic acid was observed in separated lymphocytes.
    • These membrane effects were predominantly noted in T cells.

    Conclusions:

    • The nylon wool column method causes substantial biochemical alterations in lymphocyte membrane composition.
    • The observed depletion of key lipids and sialic acid renders the method inappropriate for biochemical investigations.
    • Researchers should consider alternative methods for lymphocyte isolation when biochemical analysis is required.