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Related Experiment Videos

Calcium requirements for increased complement receptor expression during neutrophil activation.

M Berger, D L Birx, E M Wetzler

    Journal of Immunology (Baltimore, Md. : 1950)
    |August 1, 1985
    PubMed
    Summary

    Calcium ions play a crucial role in neutrophil function. Intracellular calcium release is essential for increased complement receptor 1 (CR1) expression, while complement receptor 3 (CR3) requires both intracellular and extracellular calcium for maximal expression.

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    Area of Science:

    • Immunology
    • Cell Biology
    • Biochemistry

    Background:

    • Human neutrophils exhibit increased surface expression of complement receptors CR1 and CR3 upon stimulation.
    • The precise role of calcium ions (Ca2+) in regulating this increased receptor expression has not been fully elucidated.

    Purpose of the Study:

    • To investigate the role of intracellular and extracellular calcium in the increased surface expression of CR1 and CR3 on human neutrophils.
    • To determine if the calcium requirements differ for CR1 and CR3 upregulation in response to various stimuli.

    Main Methods:

    • Utilized monoclonal antibodies and flow cytometry to quantify CR1 and CR3 surface expression on neutrophils.
    • Manipulated extracellular and intracellular calcium levels using ionophores (A23187), chelators (EDTA), and intracellular calcium release inhibitors (TMB-8).

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  • Assessed responses to stimuli including f-MLP, LTB4, and phorbol myristate acetate.

    • Main Results:

    • Increased cytosolic free Ca2+ significantly upregulated both CR1 and CR3 expression.
    • Release of intracellular Ca2+ was necessary and sufficient for CR1 upregulation by multiple stimuli.
    • Maximal CR3 upregulation required both intracellular calcium release and extracellular calcium influx, with extracellular Ca2+ enhancing the response.

    Conclusions:

    • The mechanisms regulating CR1 and CR3 surface expression differ in their calcium dependency.
    • Intracellular calcium release is critical for CR1 expression, while CR3 expression involves both intracellular and extracellular calcium.
    • These findings suggest distinct signaling pathways for the upregulation of these two important complement receptors.