Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Dimorphic-root ideotype demonstrates increased rice yield in nutrient-deficient paddy fields in Madagascar.

Journal of experimental botany·2026
Same author

Biomarker genes for model-based prediction of drought-stress perception levels in rice.

BMC plant biology·2025
Same author

Effect of heterozygous deletions on phenotypic changes and dosage compensation in Arabidopsis thaliana.

Scientific reports·2025
Same author

Detection of quantitative trait loci for rice root systems grown in paddies based on nondestructive phenotyping using X-ray computed tomography.

The Plant journal : for cell and molecular biology·2024
Same author

Hydroponic Culture of Rice Seedlings for Stress Response Assay.

Methods in molecular biology (Clifton, N.J.)·2024
Same author

Transcriptome-based prediction for polygenic traits in rice using different gene subsets.

BMC genomics·2024

Related Experiment Video

Updated: Jan 6, 2026

Multipronged Phenotyping Approaches to Characterize Sugarcane Root Systems
09:21

Multipronged Phenotyping Approaches to Characterize Sugarcane Root Systems

Published on: August 17, 2022

1.5K

Backhoe-assisted monolith method for plant root phenotyping under upland conditions.

Shota Teramoto1, Yuka Kitomi1, Ryo Nishijima2

  • 1Institute of Crop Science, National Agriculture and Food Research Organization, Tsukuba, Ibaraki 305-8518, Japan.

Breeding Science
|October 11, 2019
PubMed
Summary

A new backhoe-assisted monolith method rapidly collects intact soil root samples. This efficient technique aids in the quantitative evaluation of root system architecture (RSA) for crop improvement.

Keywords:
image analysismonolithriceroot morphologyroot sampling

More Related Videos

RGB and Spectral Root Imaging for Plant Phenotyping and Physiological Research: Experimental Setup and Imaging Protocols
11:37

RGB and Spectral Root Imaging for Plant Phenotyping and Physiological Research: Experimental Setup and Imaging Protocols

Published on: August 8, 2017

16.9K
Using Flatbed Scanners to Collect High-resolution Time-lapsed Images of the Arabidopsis Root Gravitropic Response
08:25

Using Flatbed Scanners to Collect High-resolution Time-lapsed Images of the Arabidopsis Root Gravitropic Response

Published on: January 25, 2014

12.8K

Related Experiment Videos

Last Updated: Jan 6, 2026

Multipronged Phenotyping Approaches to Characterize Sugarcane Root Systems
09:21

Multipronged Phenotyping Approaches to Characterize Sugarcane Root Systems

Published on: August 17, 2022

1.5K
RGB and Spectral Root Imaging for Plant Phenotyping and Physiological Research: Experimental Setup and Imaging Protocols
11:37

RGB and Spectral Root Imaging for Plant Phenotyping and Physiological Research: Experimental Setup and Imaging Protocols

Published on: August 8, 2017

16.9K
Using Flatbed Scanners to Collect High-resolution Time-lapsed Images of the Arabidopsis Root Gravitropic Response
08:25

Using Flatbed Scanners to Collect High-resolution Time-lapsed Images of the Arabidopsis Root Gravitropic Response

Published on: January 25, 2014

12.8K

Area of Science:

  • Agricultural Science
  • Plant Science
  • Agronomy

Background:

  • Root system architecture (RSA) is crucial for plant water and nutrient uptake.
  • Modifying RSA can enhance crop root performance.
  • Rapid and efficient root phenotyping methods are needed for crop improvement.

Purpose of the Study:

  • To introduce a novel, rapid method for collecting intact root samples from a defined soil volume.
  • To assess the efficiency and utility of the backhoe-assisted monolith technique for root phenotyping.

Main Methods:

  • A backhoe-assisted monolith method using a steel cylinder to excavate soil samples around individual rice plants.
  • Excavation of soil samples with dimensions of 20 cm width and 25 cm depth.
  • Isolation and analysis of root samples using root image analysis software.

Main Results:

  • The backhoe-assisted monolith method allowed for rapid collection of root samples, taking approximately five minutes per plant.
  • Quantitative evaluation of root traits (shallow, intermediate, deep roots) in three rice lines was successfully performed.
  • The data obtained showed trends consistent with previous findings, validating the method's effectiveness.

Conclusions:

  • The backhoe-assisted monolith method provides an efficient and practical approach for rapid root sampling.
  • This technique facilitates quantitative evaluation of root system architecture in field conditions.
  • The method holds potential for improving crop breeding programs through enhanced root phenotyping.