Cultivating river sediments into efficient denitrifying sludge for treating municipal wastewater

The river sediment contains a lot of pollutants in many cases, and needs to be treated appropriately for the restoration of water environments. In this study, a novel method was developed to convert river sediment into denitrifying sludge in a sequencing batch reactor (SBR). The river sediment was added into the reactor daily and the hydraulic retention time (HRT) of the reactor was gradually reduced from 8 to 4 h. The reactor achieved in the $$ removal efficiency of 85% with the $$ removal rate of 0.27 kg N m^-3 d^-1. Response surface analysis represents that nitrate removal was affected mainly by HRT, followed by sediment addition. The denitrifying sludge achieved the highest activity with the following conditions: $$ 50 mg l^-1, HRT 6 h and adding 6 ml river sediments to 1 l wastewater of reactor per day. As a result, the cultivated denitrifying sludge could remove 80% $$ for real municipal wastewater, and the high-throughput sequence analysis indicated that major denitrifying bacteria genera and the relative abundance in the cultivated denitrifying sludge were Diaphorobacter (33.82%) and Paracoccus (24.49%). The river sediments cultivating method in this report can not only obtain denitrifying sludge, but also make use of sediment resources, which has great application potential.