MiR-885-5p promotes gastric cancer proliferation and invasion through regulating YPEL1

Dysregulation of microRNAs (miRNAs) was found to play crucial roles in the carcinogenesis of multiple human cancers. This study was aimed to investigate the biological function of miR-885-5p and associated mechanisms in gastric cancer (GC).

Reverse Transcription-quantitative Polymerase Chain Reaction was used to measure miR-885-5p level in GC cell lines and normal cell line. The effects of miR-885-5p expression on cell proliferation, colony formation, and invasion were investigated by Cell Counting Kit-8 (CCK-8) assay, colony formation assay, and transwell invasion assay, respectively. Furthermore, Luciferase activity reporter assay and Western blot were conducted to validate Yippee-like-1 (YPEL1) as a direct target of miR-885-5p.

We found that miR-885-5p expression level was elevated in GC cell lines compared with normal cell line. Additionally, the knockdown of miR-885-5p inhibits GC cell proliferation, colony formation, and cell invasion in vitro. Notably, rescue experiments demonstrated that the knockdown of YPEL1 partially reversed the effects of miR-885-5p on GC cell growth and invasion.

The present study suggested that miR-885-5p regulates GC proliferation, colony formation, and invasion via targeting YPEL1.