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LncRNA NBR2 inhibits EMT progression by regulating Notch1 pathway in NSCLC

Y-P Gao1, Y Li, H-J Li

  • 1Department of Emergency, Shanxian Central Hospital, Heze, China. hezeshanxian@163.com.

European Review for Medical and Pharmacological Sciences
|October 11, 2019
PubMed
Abstract

Insights

Long non-coding RNA NBR2 is downregulated in non-small-cell lung cancer (NSCLC), suppressing tumor progression and epithelial-mesenchymal transition (EMT) via the Notch1 pathway.

Area of Science:

  • Oncology
  • Molecular Biology
  • Gene Regulation

Background:

  • Non-small-cell lung cancer (NSCLC) is a leading cause of cancer mortality.
  • The role of long non-coding RNAs (lncRNAs) in cancer development is an emerging area of research.
  • Understanding the molecular mechanisms underlying NSCLC progression is crucial for developing effective therapies.

Purpose of the Study:

  • To investigate the role of lncRNA NBR2 in NSCLC.
  • To elucidate the molecular mechanisms by which NBR2 influences NSCLC progression.
  • To determine the relationship between NBR2 expression and clinical features in NSCLC patients.

Main Methods:

  • Quantitative Real-Time Polymerase Chain Reaction (qRT-PCR) to assess NBR2 expression in NSCLC tissues and cells.
  • Transfection of NSCLC cells with pcDNA3.1-NBR2 vectors to study NBR2's functional effects.
  • Cell counting kit-8 (CCK-8) and Transwell assays to evaluate cell proliferation and migration.
  • Epithelial-mesenchymal transition (EMT) RT2 PCR array and Western blot to analyze EMT-related gene and protein expression, including Notch1 signaling pathway components.

Main Results:

  • LncRNA NBR2 expression was significantly decreased in NSCLC tissues compared to normal tissues.
  • Low NBR2 expression correlated with poor prognosis and larger tumor size in NSCLC patients.
  • Overexpression of NBR2 inhibited NSCLC cell viability, migration, and suppressed Notch1 and EMT-related gene expression.
  • Simultaneous overexpression of NBR2 and Notch1 partially reversed the inhibitory effects of NBR2.

Conclusions:

  • LncRNA NBR2 functions as a tumor suppressor in NSCLC.
  • NBR2 inhibits NSCLC progression by suppressing the epithelial-mesenchymal transition (EMT).
  • The Notch1 signaling pathway is a key mediator of NBR2's tumor-suppressive effects in NSCLC.

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