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RNA-seq reveals transcriptome changes of the embryonic lens cells in Prox1 tissue specific knockout mice
1Department of Ophthalmology, 4th Affiliated Hospital of China Medical University, Eye Hospital of China Medical University, Key Laboratory of Lens Research of Liaoning Province, Shenyang, China. dolphinemu@foxmail.com.
European Review for Medical and Pharmacological Sciences
|October 11, 2019
Summary
Prox1 mutations disrupt mouse lens development by altering gene expression. This study reveals key molecular pathways and transcription factors involved in lens fiber cell development, offering insights into eye genomics.
Area of Science:
- Developmental Biology
- Genomics
- Ophthalmology
Background:
- Prox1 is crucial for lens fiber cell elongation in mice.
- Understanding Prox1's role is key to deciphering lens development.
Purpose of the Study:
- To investigate the molecular mechanisms by which Prox1 mutations affect lens fiber cell development.
- To identify downstream targets and pathways regulated by Prox1 in the mouse lens.
Main Methods:
- Comparative transcriptome analysis of Prox1 conditional knockout (cKO) and wild-type (WT) mouse lenses.
- Differential gene expression analysis using R package "edgeR".
- Gene Ontology (GO) and KEGG pathway enrichment analysis, followed by protein-protein interaction (PPI) network construction using Cytoscape.
Main Results:
- Identified 2263 differentially expressed genes (DEGs) between cKO and WT lenses.
- Down-regulated genes were enriched in eye development and metabolism pathways; up-regulated genes involved Hedgehog and MAPK signaling.
- Predicted 30 transcription factors as potential downstream targets of Prox1 in mouse lens development.
Conclusions:
- Prox1 conditional knockout mice exhibit a distinct lens cell transcriptome profile.
- This study provides a valuable genomic resource for understanding mouse lens development and related disorders.
