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Related Experiment Video

Updated: Jan 6, 2026

The Use of Reverse Phase Protein Arrays RPPA to Explore Protein Expression Variation within Individual Renal Cell Cancers

The Use of Reverse Phase Protein Arrays RPPA to Explore Protein Expression Variation within Individual Renal Cell Cancers

Published on: January 22, 2013

TFEB Expression Profiling in Renal Cell Carcinomas: Clinicopathologic Correlations.

Sounak Gupta¹, Pedram Argani², Achim A Jungbluth¹

¹Departments of Pathology.

The American Journal of Surgical Pathology

|October 11, 2019

Summary

TFEB-rearranged renal tumors show higher TFEB expression than 6p21.1-amplified tumors, despite both having increased TFEB gene expression. This suggests aggressive behavior in amplified tumors may stem from co-amplified genes.

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Multiplexed Immunofluorescence Analysis and Quantification of Intratumoral PD-1+ Tim-3+ CD8+ T Cells

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Published on: February 8, 2018

Area of Science:

Oncology
Genetics
Molecular Biology

Background:

TFEB (transcription factor EB) is overexpressed in TFEB-rearranged and 6p21.1-amplified renal cell carcinomas.
Renal tumors with 6p21.1 amplification are suggested to be more aggressive than TFEB-rearranged tumors.

Purpose of the Study:

To compare relative TFEB gene expression in TFEB-rearranged versus 6p21.1-amplified renal tumors.
To investigate the correlation between TFEB expression, tumor morphology, and downstream marker activation.

Main Methods:

Analysis of 37 TFEB-altered renal tumors (15 amplified, 22 rearranged) using fluorescence in situ hybridization or comprehensive molecular profiling.
Quantification of TFEB mRNA expression via digital droplet PCR, correlated with TFEB immunohistochemistry.

Main Results:

TFEB-altered tumors exhibited significantly higher TFEB expression (168.9%) compared to controls (7%).
TFEB expression was higher in rearranged tumors (224.7%) than in amplified tumors (51.2%).
Classic biphasic morphology was exclusive to TFEB-rearranged tumors and correlated with higher TFEB expression.

Conclusions:

While both tumor types show increased TFEB expression, TFEB-rearranged tumors have higher levels than 6p21.1-amplified ones.
The aggressive behavior of 6p21.1 amplified renal tumors may be attributed to co-amplified genes (e.g., VEGFA, CCND3) rather than solely TFEB levels.
Downstream marker expression is less consistent in amplified neoplasms, supporting TFEB's differential role.